Example sentences for: taqman

How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Quantitative RT-PCR assays were performed on the ABI Prism 7900HT sequence-detection system using the Taqman Reverse Transcription Kit (Applied Biosystems) and QuantiTech SYBRGreen PCR Kit (Qiagen) using a two-step reaction with protocols supplied by manufacturers.

  • Real-time PCR was performed using Taqman Universal Polymerase Master Mix (Applied Biosystems).

  • Isolation of total RNA and synthesis of cDNA were performed as previously described [ 19 ] . RT-PCR reactions of cDNAs were conducted in a total volume of 15 μl with 2 × Taqman Master Mix (Applied Biosystems) and primers at optimized concentrations.

  • This cutoff is arbitrary, but from our experience with Taqman confirmation (data not shown), a two-fold change in expression level reliably distinguishes real signals from background noise for most expression experiments.

  • Confirmation by Taqman (Perkin-Elmer) quantitative PCR was performed for nine selected genes as described in [ 12].


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