Words similar to taqman

Example sentences for: taqman

How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:

Following the identification of regulated genes, a second method - quantitative reverse transcription polymerase chain reaction (RT-PCR) using Taqman™ - was used to establish that selected, regulated genes detected by microarray could be confirmed in the same sample by RT-PCR, to compare the magnitude of regulation determined by microarray and RT-PCR and to verify the validity of selected regulated genes by examining the gene expression levels in lung RNA from multiple, individual control and challenged monkeys.
The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.
First, the accuracy of microarray technology was tested using quantitative real-time RT-PCR (Taqman).
Quantitative RT-PCR assays were performed on the ABI Prism 7900HT sequence-detection system using the Taqman Reverse Transcription Kit (Applied Biosystems) and QuantiTech SYBRGreen PCR Kit (Qiagen) using a two-step reaction with protocols supplied by manufacturers.
The TaqMan probe carries a 5' FAM (6-carboxy-fluorescein) reporter dye and a 3' TAMRA (6-carboxy-tetramethyl-rhodamine) quencher dye (Mega Bases, Chicago, IL, USA) The quantity of cDNA of the gene of interest was directly related to the fluorescence detection of FAM after 40 cycles.