Words similar to taqman
Example sentences for: taqman
How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:
Table 4summarizes all the Taqman primers and probe information and sequences.
Real-time PCR was performed using Taqman Universal Polymerase Master Mix (Applied Biosystems).
RNA was subjected to quantitative RT-PCR analysis (Taqman; Applied Biosystems, Foster City, USA) utilizing Gli1, Ptc1 and GAPDH primers and probes.
The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.
Following the identification of regulated genes, a second method - quantitative reverse transcription polymerase chain reaction (RT-PCR) using Taqman™ - was used to establish that selected, regulated genes detected by microarray could be confirmed in the same sample by RT-PCR, to compare the magnitude of regulation determined by microarray and RT-PCR and to verify the validity of selected regulated genes by examining the gene expression levels in lung RNA from multiple, individual control and challenged monkeys.