Words similar to taqman

Example sentences for: taqman

How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:

The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.
Following the identification of regulated genes, a second method - quantitative reverse transcription polymerase chain reaction (RT-PCR) using Taqman™ - was used to establish that selected, regulated genes detected by microarray could be confirmed in the same sample by RT-PCR, to compare the magnitude of regulation determined by microarray and RT-PCR and to verify the validity of selected regulated genes by examining the gene expression levels in lung RNA from multiple, individual control and challenged monkeys.
Table 4summarizes all the Taqman primers and probe information and sequences.
Confirmation by Taqman (Perkin-Elmer) quantitative PCR was performed for nine selected genes as described in [ 12].
Isolation of total RNA and synthesis of cDNA were performed as previously described [ 19 ] . RT-PCR reactions of cDNAs were conducted in a total volume of 15 μl with 2 × Taqman Master Mix (Applied Biosystems) and primers at optimized concentrations.