Example sentences for: taqman

How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following the identification of regulated genes, a second method - quantitative reverse transcription polymerase chain reaction (RT-PCR) using Taqman™ - was used to establish that selected, regulated genes detected by microarray could be confirmed in the same sample by RT-PCR, to compare the magnitude of regulation determined by microarray and RT-PCR and to verify the validity of selected regulated genes by examining the gene expression levels in lung RNA from multiple, individual control and challenged monkeys.

  • The PCR reactions were in duplicate on an ABI PRISM 7700 Sequence detection system (Taqman™).

  • First, the accuracy of microarray technology was tested using quantitative real-time RT-PCR (Taqman).

  • Isolation of total RNA and synthesis of cDNA were performed as previously described [ 19 ] . RT-PCR reactions of cDNAs were conducted in a total volume of 15 μl with 2 × Taqman Master Mix (Applied Biosystems) and primers at optimized concentrations.

  • Table 4summarizes all the Taqman primers and probe information and sequences.


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