Words similar to taqman

Example sentences for: taqman

How can you use “taqman” in a sentence? Here are some example sentences to help you improve your vocabulary:

Isolation of total RNA and synthesis of cDNA were performed as previously described [ 19 ] . RT-PCR reactions of cDNAs were conducted in a total volume of 15 μl with 2 × Taqman Master Mix (Applied Biosystems) and primers at optimized concentrations.
Quantitative RT-PCR (Taqman™)
The PCR reaction was performed in a final volume of 50 μl using TaqMan Universal PCR Master Mix containing AmpliTaq Gold RDNA Polymerase, AmpErase RUNG, dNTPs (with dUTP), Passive Reference 1, optimized buffer components (proprietary formulation) and 1 μl of cDNA template.
First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).
The TaqMan probe carries a 5' FAM (6-carboxy-fluorescein) reporter dye and a 3' TAMRA (6-carboxy-tetramethyl-rhodamine) quencher dye (Mega Bases, Chicago, IL, USA) The quantity of cDNA of the gene of interest was directly related to the fluorescence detection of FAM after 40 cycles.