Example sentences for: taq

How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each round of PCR consisted of 31 cycles at 94°C for 20 seconds, 55°C for 40 seconds, and 72°C for 45 seconds; Platinum ®Taq DNA polymerase (GibcoBRL, Baltimore, MD) was used for DNA amplification.

  • The PCR products for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) were amplified in a 25 μl reaction containing 3 mM MgCl 2 , 10X PCR buffer supplied by Pierce (Rockford, IL), 5% DMSO, 0.2 mM dNTPs, 0.5 μM of each primer, and 0.5 U of Taq DNA polymerase supplied by Pierce [Rockford, IL].

  • To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).

  • Briefly, 3 μl of the cDNA was in a reaction of 25 μl that contained final concentrations of 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 3.5 mM MgCl 2 , 200 μM dNTP, 500 nM each primer (Mega Bases, Chicago, IL, USA), 200 nM FAM-TAMRA probe (Mega Bases, IL, USA), 100 nM Blue 636 (BD 636), 0.05 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).

  • For gene-specific amplification, 1 μl of nonspecifically amplified cDNA was amplified by PCR in 25 μ l 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 1.5 mmol/l MgCl 2 , 0.01% Triton-X, 200 μmol/l dNTP and 0.625 U Taq DNA polymerase.


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