Example sentences for: taq

How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR products for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) were amplified in a 25 μl reaction containing 3 mM MgCl 2 , 10X PCR buffer supplied by Pierce (Rockford, IL), 5% DMSO, 0.2 mM dNTPs, 0.5 μM of each primer, and 0.5 U of Taq DNA polymerase supplied by Pierce [Rockford, IL].

  • The PCR products were then purified and cut with Taq I at 65°C for 2 hr.

  • 5 μL of 10X JumpStart Taq Polymerase Reaction Buffer (Sigma, St. Louis, MO), 2.55 μL of dNTP (20 mM each), 1.5 μL of 30% BSA (Sigma), 2.55 μL 10% Tween 20, and 56.

  • PCR was performed using a standard protocol as follows with a final volume of 50 μl; 5 μl 10X PCR buffer, 5 μl 2 mM dNTP, 5 μl forward primer (concentration 20 ng/ul), 5 μl reverse primer (concentration 20 ng/μl), 1.5 μl 50 mM MgCl, 0.25 μl Taq polymerase (5 U/μl), and 1 μl sample purified genomic DNA (concentration approximately 30 ng/μl), and 5 μl dimethyl sulfoxide.

  • First, two different thermostable polymerases ( Taq and Pwo polymerase) were used to generate the primary amplification products for cloning and subsequent sequence analysis.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast