Example sentences for: taq

How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, 3 μl of the cDNA was in a reaction of 25 μl that contained final concentrations of 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 3.5 mM MgCl 2 , 200 μM dNTP, 500 nM each primer (Mega Bases, Chicago, IL, USA), 200 nM FAM-TAMRA probe (Mega Bases, IL, USA), 100 nM Blue 636 (BD 636), 0.05 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).

  • Degenerative PCR was carried out using Taq DNA Polymerase (Promega) with 5'-GATGGGAAAC(A/G)GCACCAG(C/T)(A/C)GAC-3' and 5'-GC(G/C)AG(A/G)ATGTTCCACTGCAT(G/C)AC-3' as forward and reverse primers respectively.

  • To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).

  • The PCR amplification was carried out in a total volume of 25 μl reaction mixture containing 1.5 mmol/l of MgCL 2 , 0.2 mmol/l of each dNTP, 50 ng of genomic DNA, 0.5 μm of each primer, and 1.25 IU of Taq DNA polymerase per manufacturer's instructions (Invitrogen, Carlsbad, CA).

  • The corresponding clones were amplified by using PCR with the primers having a Taq I site at the end.


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