Words similar to taq
Example sentences for: taq
How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, 3 μl of the cDNA was in a reaction of 25 μl that contained final concentrations of 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 3.5 mM MgCl 2 , 200 μM dNTP, 500 nM each primer (Mega Bases, Chicago, IL, USA), 200 nM FAM-TAMRA probe (Mega Bases, IL, USA), 100 nM Blue 636 (BD 636), 0.05 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).
Degenerative PCR was carried out using Taq DNA Polymerase (Promega) with 5'-GATGGGAAAC(A/G)GCACCAG(C/T)(A/C)GAC-3' and 5'-GC(G/C)AG(A/G)ATGTTCCACTGCAT(G/C)AC-3' as forward and reverse primers respectively.
To amplify the cDNA non-specifically, PCR was performed with 100 μl of 10 mmol/l Tris-HCl (pH 9.0), 50 mmol/l KCl, 2.5 mmol/l MgCl 2 , 0.01% Triton-X, 1 mmol/l dNTP, 10 U Taq DNA polymerase (Promega), and 2 μmol/l X-(dT) 24 primer (ATG TCG TCC AGG CCG CTC TGG ACA AAA TAT GAA TTC-dT 24 ; Integrated DNA Technologies Incorporated).
The PCR amplification was carried out in a total volume of 25 μl reaction mixture containing 1.5 mmol/l of MgCL 2 , 0.2 mmol/l of each dNTP, 50 ng of genomic DNA, 0.5 μm of each primer, and 1.25 IU of Taq DNA polymerase per manufacturer's instructions (Invitrogen, Carlsbad, CA).
The corresponding clones were amplified by using PCR with the primers having a Taq I site at the end.