Words similar to taq
Example sentences for: taq
How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RT reaction was incorporated into PCR, carried out using a mixture of Taq (Roche) and Pfu (Stratagene) polymerases (20:1) to ensure high fidelity [ 50 ] . Elongation in PCR was performed at 62°C.
Afterward, 5U Taq DNA polymerase was added, followed by an additional 25 cycles of PCR.
The PCR mix per reaction included 10 μl SYBR Green buffer, 1.5 mM MgCl 2 , 1 μM dNTP mix (with dUTP), 0.5 μl CMV primer mix, 2.5 units Ampli Taq gold, 0.05 units of uracil-N-glycosylase (UNG, Epicentre, Madison, WI) that had been diluted 1:10 in UNG buffer, and molecular grade water to bring the volume up to 45 μl with the addition of 5 μl of cDNA or standard.
In this size range the Stoffel fragment of Taq polymerase yielded a better S/N than Taq polymerase.
First, two different thermostable polymerases ( Taq and Pwo polymerase) were used to generate the primary amplification products for cloning and subsequent sequence analysis.