Words similar to taq

Example sentences for: taq

How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:

Full-length ds-cDNA was synthesized by adding 57 μl nuclease-free water, 10 μl 10× PCR buffer (Roche), 10 μl 25 mM MgCl 2 , 1 μl 10 mM dNTP and 5 u Ampli-Taq Gold DNA Polymerase (Roche).
The entire mutated cDNA was sequence verified and subcloned into a fresh pcDNA3 vector to avoid non-specific Taq DNA polymerase errors.
The RT reaction was incorporated into PCR, carried out using a mixture of Taq (Roche) and Pfu (Stratagene) polymerases (20:1) to ensure high fidelity [ 50 ] . Elongation in PCR was performed at 62°C.
Briefly, 3 μl of the cDNA was in a reaction of 25 μl that contained final concentrations of 1× Platinum PCR buffer (Gibco/Life Technologies, Grand Island, NY, USA), 3.5 mM MgCl 2 , 200 μM dNTP, 500 nM each primer (Mega Bases, Chicago, IL, USA), 200 nM FAM-TAMRA probe (Mega Bases, IL, USA), 100 nM Blue 636 (BD 636), 0.05 units platinum Taq DNA polymerase (Gibco/Life Technologies, Grand Island, NY, USA).
Amplification reactions consisted of a 25 μl reaction containing 100 ng genomic DNA or 20 ng BAC DNA, 200 μM dNTPs, 200 nM each forward and reverse primer, 1 × reaction buffer (10 mM Tris-HCl pH 8.3, 50 mM KCl), 1.5 mM MgCl 2 , and 1 U Maxi Taq polymerase [ 36].