Words similar to taq
Example sentences for: taq
How can you use “taq” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR products for primer set cht3(f) and cht3(r) and primer set Antonia LTR(f) and Antonia LTR(r) were amplified in a 25 μl reaction containing 3 mM MgCl 2 , 10X PCR buffer supplied by Pierce (Rockford, IL), 5% DMSO, 0.2 mM dNTPs, 0.5 μM of each primer, and 0.5 U of Taq DNA polymerase supplied by Pierce [Rockford, IL].
The PCR products were then purified and cut with Taq I at 65°C for 2 hr.
5 μL of 10X JumpStart Taq Polymerase Reaction Buffer (Sigma, St. Louis, MO), 2.55 μL of dNTP (20 mM each), 1.5 μL of 30% BSA (Sigma), 2.55 μL 10% Tween 20, and 56.
PCR was performed using a standard protocol as follows with a final volume of 50 μl; 5 μl 10X PCR buffer, 5 μl 2 mM dNTP, 5 μl forward primer (concentration 20 ng/ul), 5 μl reverse primer (concentration 20 ng/μl), 1.5 μl 50 mM MgCl, 0.25 μl Taq polymerase (5 U/μl), and 1 μl sample purified genomic DNA (concentration approximately 30 ng/μl), and 5 μl dimethyl sulfoxide.
First, two different thermostable polymerases ( Taq and Pwo polymerase) were used to generate the primary amplification products for cloning and subsequent sequence analysis.