Words similar to targeting
Example sentences for: targeting
How can you use “targeting” in a sentence? Here are some example sentences to help you improve your vocabulary:
Once the targeting construct is in hand it takes approximately four to five weeks to generate transgenic mouse embryos.
pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.
A PCR based gene targeting approach [ 61 62 ] was used to delete the rec12 +coding region and replace it with the ura4 +gene.
The targeting plasmid used to delete hprt exon1 was called pΔHprt to indicate this deletion.
Cre-mediated targeting of single copy transgenes to specific genomic sites that have previously been marked by loxP elements has also been described both in somatic cells and more recently in ES cells using a double LoxP targeting strategy [ 22, 23].
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