Words similar to synthesis
Example sentences for: synthesis
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Regulation of EPK function can occur at many levels, including control of synthesis, posttranslational modification, binding of regulatory proteins and subcellular localization.
Second strand cDNA synthesis was performed by mixing the first strand synthesis reaction with 106 μl RNase-free water, 15 μl 10X Advantage™ PCR buffer (Clontech), 3 μl 10 mM dNTP mix, 3 μl Advantage™ cDNA polymerase mix (Clontech), and 1 μl RNase H (2 U/μl, Invitrogen™).
MC57 cells, which demonstrated similar changes in cell adhesion to RAW cells following IDO expression, exhibited lower levels of COX-2 synthesis upon IDO expression and lower levels of PG E 2 relative to other PGs such as D 2 . Furthermore, adding back PGE 2 to IDO-expressing MC57 cells did not reproduce the wild type phenotype (not shown).
The second class, GSII, is found in all eukaryotes and a small number of prokaryotes, and appears to be involved in purine synthesis [ 35 ] . Unlike GSI, GSII is not adenylylated (and lacks the conserved tyrosine).
Binding of free E2F/DP heterodimers to E2F sites generally activates transcription of proteins required for G1 → S transition and DNA synthesis [ 1, 2, 3, 4, 5], whereas complex formation with pRb or other pocket proteins including p107 and pRb-2/p130 silences transcriptional activities of downstream target genes [ 6, 7, 8, 9, 10].