Words similar to sulfo-

Example sentences for: sulfo-

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Biotinylation of adenoviral vectors with sulfo-NHS-LC-biotin
Sulfo-NHS-LC-biotin (Pierce Chemical) was dissolved in dimethylformamide at a concentration of 3 mg/ml and added to Ad5.CMV-LacZ [1.
Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.
When Ad5.CMV-LacZ was treated with sulfo-NHS-LC-biotin at a concentration of 0.6 μg/ml or lower, almost no infection of D-17 cells was seen.
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.