Words similar to sulfo-
Example sentences for: sulfo-
How can you use “sulfo-” in a sentence? Here are some example sentences to help you improve your vocabulary:
When Ad5.CMV-LacZ was treated with sulfo-NHS-LC-biotin at a concentration of 0.6 μg/ml or lower, almost no infection of D-17 cells was seen.
Ad5.CMV-LacZ was treated at varying concentrations (0 - 100 μg/ml) of sulfo-NHS-LC-biotin on ice for 2 h in PBS (pH 7.4), followed by the removal of non-virion-associated biotinylation reagent by ultrafiltration.
First, spots of biotin moieties on the wells were created by using sulfo-NHS-LC-biotin.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.
This procedure is explained in detail in Coonrod et al, 1999 [ 8 ] . Briefly, after collection zona-free eggs were washed six times in BWW medium (Irvine Scientific, Santa Ana, CA) containing 100 μg/ml polyvinylalcohol (PVA, Sigma, St. Louis, MO), biotinylated with 2 mg/ml Sulfo-NHS biotin (Pierce, Rockford, IL) in BWW/PVA for 7 min at room temperature, and washed six times in BWW/PVA.