Words similar to sulfo-

Example sentences for: sulfo-

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Varying numbers of biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin, were applied to streptavidin-coated wells (well diameter, 0.64 cm) for immobilization, followed by the removal of unbound viral particles.
Little effect on viral infectivity was seen when the adenoviral vectors were treated with sulfo-NHS-LC-biotin at concentrations up to approximately 40 μg/ml (data not shown).
After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.
Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.
The maximum infectivity was seen with Ad5.CMV-LacZ that had been treated with 15 μg/ml sulfo-NHS-LC-biotin, where the infectivity was approximately 75% of that of unmodified Ad5.CMV-LacZ used free in solution.