Words similar to sulfo-
Example sentences for: sulfo-
How can you use “sulfo-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The maximum infectivity was seen with Ad5.CMV-LacZ that had been treated with 15 μg/ml sulfo-NHS-LC-biotin, where the infectivity was approximately 75% of that of unmodified Ad5.CMV-LacZ used free in solution.
Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.
First, spots of biotin moieties on the wells were created by using sulfo-NHS-LC-biotin.
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and added to streptavidin-coated wells (2.
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