Words similar to sulfate

Example sentences for: sulfate

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Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .
FtsZ was purified from the bacterial supernatant by discarding a precipitate at 20% saturated ammonium sulfate, then precipitating the remaining FtsZ by raising the ammonium sulfate level to 25% saturation [ 14].
The resulting cleared supernatant was brought to 30% saturation with powdered ammonium sulfate and incubated with stirring for 30 min.
In contrast, when tissue disruption was significant and accompanied by glial scarring, including astrogliosis and the upregulation of chondroitin sulfate proteoglycans (CSPGs), axonal growth stopped in areas of CSPG expression.
The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.