Example sentences for: subtractive

How can you use “subtractive” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Some of the genes identified by DADA including MCP-2 and Cystatin C were neither discovered by means of subtractive hybridization [ 3 ] , nor by differential display [ 9 ] , although these methods were successfully used in comparable settings in our laboratory and lead to a high number of differentially expressed genes.

  • Suppression subtractive hybridization (SSH)

  • Suppression Subtractive Hybridization

  • There are several strategies for identifying differentially expressed transcripts, including differential display (DD), representational difference analysis (RDA), serial analysis of gene expression (SAGE), enzymatic degradation subtraction and subtractive hybridization [ 29 30 31 32 33 ] . We chose the PCR-based suppression subtractive hybridization (SSH) procedure for several reasons: it includes a normalization step, it enriches for differentially expressed transcripts, and it yields cDNA fragments that can be used directly for the construction of DNA microarrays.

  • SSH was carried out using the PCR-Select Subtractive Hybridization kit (Clontech, Palo Alto, CA).


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