Example sentences for: subtractive

How can you use “subtractive” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The subtractive hybridization was performed by adding 1.5 μl driver cDNA to each tube, one containing 1.5 μl of adaptor 1 and the other containing 1.5 μl adapter 2R-ligated tester cDNA (tester cDNA was approximately 30 times less concentrated than driver cDNA) in 1 μl 4X hybridization buffer.

  • Animals used in subtractive hybridization (from both sexual and asexual strains) were generously provided by Dr. Sheiman I.M. (Institute of Biophysics, Pushino).

  • Some of the genes identified by DADA including MCP-2 and Cystatin C were neither discovered by means of subtractive hybridization [ 3 ] , nor by differential display [ 9 ] , although these methods were successfully used in comparable settings in our laboratory and lead to a high number of differentially expressed genes.

  • RNAs isolated from these cultures were then subjected to suppression subtractive hybridization and differential display (SSHDD).

  • Two primary methods were used to compare differences in gene expression between wild-type and mutant infections; direct subtractive comparisons of mutant from wild type, and SAM analysis of the 2- and 4-hour time points as single classes.


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