Words similar to subtractive
Example sentences for: subtractive
How can you use “subtractive” in a sentence? Here are some example sentences to help you improve your vocabulary:
The eluted phage were then panned vs. other members of the TNF family as a way to obtain peptides specific to the original target (Subtractive panning).
Representational difference analysis (RDA), a DNA subtractive hybridization method [ 18 ] , was used in the present study to test the working hypothesis that, to achieve the metastatic state, primary mammary carcinoma cells must acquire genetic changes in addition to those changes that led to transformation.
There are several strategies for identifying differentially expressed transcripts, including differential display (DD), representational difference analysis (RDA), serial analysis of gene expression (SAGE), enzymatic degradation subtraction and subtractive hybridization [ 29 30 31 32 33 ] . We chose the PCR-based suppression subtractive hybridization (SSH) procedure for several reasons: it includes a normalization step, it enriches for differentially expressed transcripts, and it yields cDNA fragments that can be used directly for the construction of DNA microarrays.
Thus, at the time points used in this study, many of the genes detected were likely regulated by these cytokines as well as Con A. In a similar vein, Herblot et al [ 42 ] used a subtractive approach to identify 66 genes induced by IL-2 but not IL-4 in a murine T cell line.
Subsequently, Zipfel and co-workers employed subtractive hybridization to isolate and differentially clone immediate/early genes activated via the T cell antigen receptor in the presence of cycloheximide [ 27 ] . A total of 60 novel TCR-induced immediate/early genes were isolated, but most were not identified at the time, in that DNA cloning and sequencing methods were tedious and time consuming.
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