Example sentences for: subtraction

How can you use “subtraction” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 32P-labeled phosphohistone was made as described [ 14 15 18 ] . When using phosphohistone as substrate, reactions were carried out in 80 μl followed by quantification of the liberated 32Pi by phosphomolybdate extraction assay [ 14 15 18 ] . Reactions were followed with time, and results were corrected by subtraction of the corresponding values from an enzyme-free reaction.

  • The liberated 32P was quantitated by a phosphomolybdate extraction assay as described previously [ 14 ] . Reactions were followed with time, and results were corrected by subtraction of the corresponding values from an enzyme-free reaction.

  • cDNA prepared from the treated samples was used as the 'tester' and that from the control sample as 'driver' for the forward subtraction to isolate fragments corresponding to genes whose expression level was increased following the treatment.

  • Recently, we supplemented the well-known SSH #(suppression subtractive hybridization) technology [ 7 8 ] with the Mirror Orientation Selection method (MOS) for eliminating background clones from SSH-generated libraries [ 9 ] . This innovative procedure allowed the application of these techniques in the subtraction of large eukaryotic genomes, including those of freshwater planarian (haploid genome size of G. tigrina is 1.4 × 10 9bp [ 10 ] ). Using the SSH-MOS combination, genomes of two different strains of G. tigrina were compared.

  • The samples were manually prepared and the data was posted with baseline subtraction.


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