Example sentences for: spectrophotometry

How can you use “spectrophotometry” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protein amounts were measured using a BCA assay kit (Pierce, #23227) and spectrophotometry.

  • RNA was extracted using Trizol reagent (Gibco BRL) and the amount of RNA was quantitated by UV spectrophotometry.

  • Since etiolation results in rapid elongation of seedlings it was expected that there would be a concomitant increase in subunit A messenger RNA as seen with proteolipid subunit mRNA in A. thaliana [ 36 ] and subunit A message levels in rapidly expanding cotton ( Gossypium hirsutum ) fibers [ 73 ] . Equivalent loading of whole RNA was evaluated via spectrophotometry measuring absorption at 260 nm using a Perkin-Elmer Lambda-3 spectrophotometer and by visual confirmation using non denaturing ethidium bromide stained agarose gel electrophoresis (results not shown).

  • Eluted fractions were evaluated by spectrophotometry and immunoblot analysis [ 42 ] . Fractions with the highest absorbance at 280 nm and the strongest reactivity by immunoblot were neutralized in 1 M Tris-HCl (pH 9.5) buffer and stored at 4°C.

  • Nucleic acids were quantitated by spectrophotometry and via inspection of ethidium bromide stained agarose gels.


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