Words similar to spectrophotometer

Example sentences for: spectrophotometer

How can you use “spectrophotometer” in a sentence? Here are some example sentences to help you improve your vocabulary:

Serial dilutions of the proteases were made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl 2 . Enzyme activity was measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA (Bachem Bioscience, Torrance, CA), 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer.
Immediately prior to PCR analysis, the methanol preserved cells were diluted in distilled water to a spectrophotometer OD 600 reading of approximately 0.2 - 0.15 Absorbance units.
Hypothalamic proopiomelanocortin (POMC) and agouti-related peptide (AGRP) mRNA and pituitary POMC mRNA were measured by Northern blot analysis as previously described [ 6 ] . Briefly, total RNA was extracted in TRIzol (GIBCO BRL, Gaithersburg, MD) and three micrograms of total RNA from hypothalamus, estimated by spectrophotometer and verified by gel electrophoresis, was subjected to Northern blot analysis to measure POMC and AGRP mRNA.
18MV spectrophotometer, using an excitation wavelength of 295 nm and a WG320 nm cut-off filter on the photomultiplier.
The concentration of total RNA and poly(A) +RNA was determined using a GeneSpec I spectrophotometer (Hitachi) and the integrity of total RNA and poly(A) +RNA was assessed using a 2100 Bioanalyzer (Agilent).