Example sentences for: spectrophotometrically

How can you use “spectrophotometrically” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The concentration and purity of RNA were quantified spectrophotometrically by measuring A 260 and A 280 ; the ratio A 260 / A 280 of pure RNA is approximately 1.8.

  • On the 6 thday, XTT dye (1 mg/ml) was added to the cells, Intracellular formazan was determined spectrophotometrically [ 18 19 ] . Similar experiments were done with HIV-1 BaL, except that PM1 cells were used instead of MT-2 cells, and virus production was measured by ELISA for p24 antigen one week after infection.

  • Ribozyme transcripts were quantified spectrophotometrically.

  • Alkaline phosphatase activity in the supernatant was determined spectrophotometrically at 405 nm wavelength as the amount of p-nitrophenol released from the substrate p-nitrophenyl phosphate (ALP10, Sigma).

  • Phosphocellulose-purified tubulin (PC-tubulin) free of microtubule-associated proteins (MAPs) was obtained by warm/cold polymerization/depolymerization with the addition of a final phosphocellulose chromatography step to separate tubulin from MAPs [ 37 38 ] . Tubulin concentrations were determined spectrophotometrically, using ε 278 = 1.2 L/(g cm) [ 39 ] .


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