Example sentences for: spectrophotometrically

How can you use “spectrophotometrically” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Alkaline phosphatase activity in the supernatant was determined spectrophotometrically at 405 nm wavelength as the amount of p-nitrophenol released from the substrate p-nitrophenyl phosphate (ALP10, Sigma).

  • Malondialdehyde and other products of lipid peroxidation can be estimated spectrophotometrically at 535 nm after reaction with thiobarbituric acid to obtain an index for lipid peroxidation [ 23 ] . The absorbance values obtained were compared against a standard curve of known concentrations of malondialdehyde and normalized to the protein content of the specimen.

  • Phosphocellulose-purified tubulin (PC-tubulin) free of microtubule-associated proteins (MAPs) was obtained by warm/cold polymerization/depolymerization with the addition of a final phosphocellulose chromatography step to separate tubulin from MAPs [ 37 38 ] . Tubulin concentrations were determined spectrophotometrically, using ε 278 = 1.2 L/(g cm) [ 39 ] .

  • This was monitored spectrophotometrically at 274 nm using an extinction coefficient of 2,630 M/cm.

  • NO production was measured by the Greiss reaction (Promega, Madison, USA), which spectrophotometrically detects nitrite, a stable breakdown product of NO whose accumulation reflects NO production.


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