Words similar to sp-a
Example sentences for: sp-a
How can you use “sp-a” in a sentence? Here are some example sentences to help you improve your vocabulary:
PD98059 had no effect on the insulin inhibition of SP-A mRNA levels.
The effects of the PI 3-kinase and the MAPK pathway inhibitors on SP-A gene expression were evaluated using two experimental models, a human immortalized epithelial cell line (H441 cells) and human fetal lung explants.
Wortmannin is a fungal metabolite that is a relatively selective inhibitor of PI 3-kinase with a half-maximal inhibition (IC 50 ) less than 5 nM [ 11 ] . In the wortmannin experiments, media were changed every 2 hours for a total of 12 hours in all of the conditions because wortmannin is unstable in solution at physiological pH [ 19 ] . The shorter incubation time with insulin in the wortmannin experiments resulted in a decreased inhibitory effect of insulin, i.e., an ~22% inhibition of SP-A mRNA levels (Fig.
However wortmannin, at concentrations of 5-200 nM, abolished the insulin-mediated inhibition of SP-A mRNA levels at all concentrations tested (5-200 nM, Fig.
Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.
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