Example sentences for: sp-a

How can you use “sp-a” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The rate of SP-A gene transcription increased in a time-dependent manner (Fig.

  • Insulin causes a time- and dose-dependent inhibition of SP-A gene expression with maximum effect at 0.25-2.

  • Nytran membranes with immobilized cDNAs for the BlueScript vector, human SP-A and human α-actin were prehybridized in 1 ml of buffer (50% formamide, 5 X SSC, 5 X Denhardt's solution, 100 μg/ml denatured herring sperm, 0.1% SDS) for 4 hours at 45°C and then hybridized to the labeled RNAs (6 × 10 6cpm in the presence of 500 units of RNasin) in duplicate for an additional 60 hours.

  • H441 cells were harvested and total RNA isolated as described previously [ 16 ] . Equal amounts of total RNA from each condition were separated on agarose gels, transferred to Nytran-plus membranes (Schleicher and Schuell, Keene, NH, USA), and subsequently hybridized with a radiolabeled human SP-A cDNA.

  • We found that wortmannin and rapamycin had no effect on basal SP-A mRNA levels, at any concentration tested.


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