Example sentences for: sp-br

How can you use “sp-br” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 2 , 0.001% gelatin, 1 mM of each dNTP, 5 mM dithiothreitol, 2.9 U RNasin, 20 pmoles of primer SP-BR (5'-GAATCACAGCTTGGACCCGC-3'), and 16 U MMLV reverse transcriptase.

  • Four PCR products were thus generated: RATPase6F-T7 adapter, SP6 adapter-RATPase6R, SP-BF-T7 adapter, and SP6 adapter-SP-BR.

  • The RATPase6F-T7 adapter and SP-BF-T7 adapter PCR products were used as templates for in vitro transcription to produce antisense riboprobes, and the SP6 adapter-RATPase6R or SP6 adapter-SP-BR PCR PCR products were used to make sense probes.

  • Similarly, a 202 bp fragment was amplified from pCR21/4SP-b with primers SP-BF and SP-BR (see above) to produce SP-B riboprobes.

  • The ligation products were amplified with an adapter-specific primer and either the probe-specific forward primer RATPase6F or SP-BF (for the T7 adapter ligation) or the probe-specific reverse primer RATPase6R or SP-BR (for the SP6 adapter ligation).


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