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Example sentences for: solution
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5 mg/ml) were prepared with fibroblasts harvested at confluence and resuspended to 10 6cells/ml collagen solution (see also [ 36 ] ). One ml of this cell suspension was added per well of a 6 well plate (Nunc).
0 in the absence of chelator and will precipitate out of solution as [VO(OH) 2 ] n . The rate of precipitation depends on the abundance and affinity of the chelator.
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
Subsequent SDS-PAGE analysis of pR samples that had been stored for periods of time up to several months indicate that after sitting for several weeks in octylglucoside solution at 4°C, the largest post-translational modification on wild-type pR is eliminated - presumably hydrolysed off of the cysteine(s) - leaving only a 31,000-MW band indistinguishable from that seen for pR-TCM (data not shown).
7% EtOH solution).
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