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Example sentences for: solution

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Cy3- and Cy5-labeled cDNA targets were then purified on QIAquick PCR purification columns, combined and concentrated by lyophilization, and hybridized to the microarray at 42°C for 16 h in hybridization solution containing 50% formamide, 5x SSC, 0.1% SDS, 20 μg mouse Cot-1 DNA and 10 μg poly(dA).
For comparison, the same numbers of unmodified Ad5.CMV-LacZ were used free in solution.
Preparation of VO 2+solution
A LPS stock of 1 mg/ml was prepared in a 0.9% sodium chloride nonpyrogenic solution from Baxter Healthcare Corp. (Toronto, ONT, Canada) and then diluted with DMEM plus 10% FBS plus P and S to the appropriate concentration used in our experiments.
For calcium-free condition, whole oocytes were rinsed three times with calcium-free frog Ringer's solution (96 mM NaCl, 2 mM KCl, 2 mM EGTA, 1 mM MgCl 2 , 5 mM HEPES, pH 7.6) prior to incubation with or without 10-20 μM capsaicin at room temperature for 30 minutes.