Words similar to solubilization

Example sentences for: solubilization

How can you use “solubilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

After washing with PBS at pH 7.4, pellets were lyzed in solubilization buffer containing 50 mM TRIS at pH 8, 1% Tween 20, 10 mM phenylmethylsulphonyl fluoride, diethyldithiocarbamic acid, leupeptin and pepstatin A (all from Sigma Chemical), sonicated, boiled with gel loading buffer (62.
The bead pellets were then washed 3 times with ice cold PBS before solubilization in SDS-PAGE sample buffer.
Crude extracts were usually diluted 1:1 (v/v) with solubilization buffer and heated to 100°C for 10 min.
After a five-minute opioid or EGF incubation, the cell monolayers were rinsed twice with ice cold phosphate-buffered saline (PBS), and the cells were solubilized by the addition of 300 μl of solubilization buffer (50 mM Tris-HCl/Base (pH7.
Solubilization in detergent presumably leads to structural distortions of the native protein conformation, and therefore a change in the absorbance properties of the chromophore.