Example sentences for: solubilization

How can you use “solubilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Solubilization in detergent presumably leads to structural distortions of the native protein conformation, and therefore a change in the absorbance properties of the chromophore.

  • After washing with PBS at pH 7.4, pellets were lyzed in solubilization buffer containing 50 mM TRIS at pH 8, 1% Tween 20, 10 mM phenylmethylsulphonyl fluoride, diethyldithiocarbamic acid, leupeptin and pepstatin A (all from Sigma Chemical), sonicated, boiled with gel loading buffer (62.

  • The bead pellets were then washed 3 times with ice cold PBS before solubilization in SDS-PAGE sample buffer.

  • After a five-minute opioid or EGF incubation, the cell monolayers were rinsed twice with ice cold phosphate-buffered saline (PBS), and the cells were solubilized by the addition of 300 μl of solubilization buffer (50 mM Tris-HCl/Base (pH7.

  • 100 μl MTT labelling reagent were added to each well and incubated for 4 hrs, followed by addition of 1 ml solubilization solution into each well.


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