Example sentences for: solubilization

How can you use “solubilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing with PBS at pH 7.4, pellets were lyzed in solubilization buffer containing 50 mM TRIS at pH 8, 1% Tween 20, 10 mM phenylmethylsulphonyl fluoride, diethyldithiocarbamic acid, leupeptin and pepstatin A (all from Sigma Chemical), sonicated, boiled with gel loading buffer (62.

  • Crude extracts were usually diluted 1:1 (v/v) with solubilization buffer and heated to 100°C for 10 min.

  • Nuclear extract from Sertoli cells (12 × 10 6) were prepared by solubilization of nuclei in 0.4 M NaCl as described previously [ 17 ] .

  • Solubilization in detergent presumably leads to structural distortions of the native protein conformation, and therefore a change in the absorbance properties of the chromophore.

  • 100 μl MTT labelling reagent were added to each well and incubated for 4 hrs, followed by addition of 1 ml solubilization solution into each well.


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