Words similar to solubilization
Example sentences for: solubilization
How can you use “solubilization” in a sentence? Here are some example sentences to help you improve your vocabulary:
After a five-minute opioid or EGF incubation, the cell monolayers were rinsed twice with ice cold phosphate-buffered saline (PBS), and the cells were solubilized by the addition of 300 μl of solubilization buffer (50 mM Tris-HCl/Base (pH7.
After washing with PBS at pH 7.4, pellets were lyzed in solubilization buffer containing 50 mM TRIS at pH 8, 1% Tween 20, 10 mM phenylmethylsulphonyl fluoride, diethyldithiocarbamic acid, leupeptin and pepstatin A (all from Sigma Chemical), sonicated, boiled with gel loading buffer (62.
100 μl MTT labelling reagent were added to each well and incubated for 4 hrs, followed by addition of 1 ml solubilization solution into each well.
Nuclear extract from Sertoli cells (12 × 10 6) were prepared by solubilization of nuclei in 0.4 M NaCl as described previously [ 17 ] .
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.
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