Example sentences for: solubilization

How can you use “solubilization” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing with PBS at pH 7.4, pellets were lyzed in solubilization buffer containing 50 mM TRIS at pH 8, 1% Tween 20, 10 mM phenylmethylsulphonyl fluoride, diethyldithiocarbamic acid, leupeptin and pepstatin A (all from Sigma Chemical), sonicated, boiled with gel loading buffer (62.

  • Protein extracts were prepared by lysing sperm or homogenized tissues with a solubilization buffer (62.

  • Crude extracts were usually diluted 1:1 (v/v) with solubilization buffer and heated to 100°C for 10 min.

  • Previous authors preferred to use 1% Triton X-100, a relatively high concentration of detergent, because it is critical for rapid solubilization of the cell membrane and cessation of cell activity.

  • 100 μl MTT labelling reagent were added to each well and incubated for 4 hrs, followed by addition of 1 ml solubilization solution into each well.


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