Example sentences for: solubilized

How can you use “solubilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After a five-minute opioid or EGF incubation, the cell monolayers were rinsed twice with ice cold phosphate-buffered saline (PBS), and the cells were solubilized by the addition of 300 μl of solubilization buffer (50 mM Tris-HCl/Base (pH7.

  • The cells were washed five times in PBS, solubilized, the DNA precipitated with 10% trichloroacetic acid at 4°C, and counted in an scintillation counter.

  • Hybrid toxin 4109 consisting of domains I and II of Cry1Aa and domain III of Cry1Ac was constructed as previously described [ 3 ] . Force-feeding bioassays on B. mori and BBMV binding assays were conducted as described [ 4 ] . Crystal proteins were solubilized and trypsinized, and active toxins were column purified as carried out previously [ 19 ] .

  • The formazan product was solubilized by addition of 100 μl of dimethyl sulfoxide (DMSO) and 100 μl of 10% SDS (in 0.01 M HCl) for 16 hours at 37°C.

  • Monolayers were then washed with cold PBS, pH 7.5, and solubilized in 1.0 ml of cell extraction buffer (50 mM HEPES, pH 7.5, 1% Triton X-100, 0.1% SDS, 0.25% deoxycholate, 150 mM sodium chloride, 1 mM EDTA, and protease inhibitors.


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