Example sentences for: solubilized

How can you use “solubilized” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were rinsed with PBS and solubilized in a commercial mammalian extraction buffer (M-Per™; Pierce, Rockford, IL) supplemented with a protease inhibitor cocktail (Complete; Roche) followed by centrifugation at 12,000 × g for 5 min.

  • After a five-minute opioid or EGF incubation, the cell monolayers were rinsed twice with ice cold phosphate-buffered saline (PBS), and the cells were solubilized by the addition of 300 μl of solubilization buffer (50 mM Tris-HCl/Base (pH7.

  • The nuclei were washed, and protein associated with the nuclei as well as protein in the supernatants was solubilized in SDS sample buffer, resolved by SDS-PAGE, and analyzed by protein immunoblotting with anti-HA antibody.

  • After incubation, tissues were removed from the rods, placed in scintillation vials and solubilized in TS-2 tissue solubilizer (Research Products International, Mount Prospect, IL).

  • A chromophore absorption maximum near 540 nm was also obtained by using difference spectroscopy of pR in crude E. coli membranes solubilized in OG (spectrum not shown).


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