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Example sentences for: smi-

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The RT-97 mAB [ 9 ] has been characterized as recognizing phosphorylated epitopes on the 210 kDa NF-H peptide [ 15 ] , and used similarly to SMI-31 to identify neurites in vitro and in situ [ 16 17 18 ] . One would predict, therefore, that labeling with RT97 would produce staining patterns, including nuclear, similar to those of SMI-31 in SH-SY5Y cells.
We asked the following questions: 1) are the immuno-labeled structures within the nucleus or just closely associated; 2) is the phosphoepitope labeled by SMI-31 and RT-97 mABs specific to NFs or can it be identified on other IFs in other cell types; and 3) is there a relationship between the cell cycle as determined by DNA synthesis and the amount of nuclear labeling by SMI-31 and RT-97?
Cross reactivity of both SMI-31 and RT-97 on glial cytoplasm and nuclei indicates the epitope is not cell type specific.
As a previous study [ 13 ] had suggested nuclear staining by antibodies targeted to cytoplasmic proteins might be fixation dependent [ 4 ] or an artifact of fixation, we did a preliminary comparison of SMI-31 labeling of methanol vs. paraformaldehyde fixed cells.
Western analysis with anti-GFAP pAB, SMI-31 mAB, RT-97 mAB, and anti-lamins mAB was performed on F98 cell extracts subjected to SDS-PAGE.