Words similar to smg
Example sentences for: smg
How can you use “smg” in a sentence? Here are some example sentences to help you improve your vocabulary:
E15 SMG (mostly Canalicular Stage) primordia were cultured using a modified Trowell method as previously described [ 13 ] . The medium consisted of BGJb (Life Technologies, Rockville, MD) supplemented with 0.5 mg ascorbic acid/ml and 50 units penicillin/streptomycin (Life Technologies), pH 7.2, and replicate cultures were changed every other day.
In addition, since TNF/TNFR1 signaling has been shown to induce embryonic SMG cell proliferation and inhibit apoptosis in vitro [ 13 ] and TNF/TNFR1 signal transduction primarily signals by induction of NF-κB nuclear translocation [ 36 ] , we postulated that TNF supplementation should have no inductive effect on SN50-treated SMGs.
This modified expression of genes and proteins associated with the inhibition of the cell cycle and the induction of apoptosis, as well as the increased activation of proapoptotic and compensatory antiapoptotic pathways, provides a "snapshot" of the broad primary and secondary effects of NF-κB signaling during SMG development.
To determine which key pathways were activated, Western blot analyses of phosphorylated or cleaved proteins in E15+2 CONT and SN50-treated explants were conducted as previously described [ 5 ] . For this set of experiments, we first determined the specificity for each of the following antibodies purchased from Cell Signaling Technology (Beverly, MA) using E15 and E17 SMG homogenates: anti-phosphorylated Erk1/2 [phospho-p44/42 MAP kinase (Thr202/Tyr204)] antibody, anti-phosphorylated c-Raf(Ser259) antibody, anti-cleaved Caspase 3 (D 175) antibody, and anti-cleaved PARP (D214) antibody.
To further demonstrate that SN50 treatment inhibited NB-κB activation, we evaluated if TNF supplementation would induce NF-κB translocation and SMG morphogenesis.
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