Words similar to smgs
Example sentences for: smgs
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Embryos were dissected in cold phosphate buffered saline (PBS) and staged according to Theiler [ 68 ] . SMGs were dissected and cultured, processed for histology, or stored at -70°C.
This increased activity of proapoptotic (caspase3/PARP) and compensatory antiapoptotic (ERK1/2) pathways is consistent with the dramatic cell death seen in SN50-treated SMGs.
However, others have shown that lower doses ≤ 100 μg/ml of SN50 specifically inhibited NF-κB nuclear translocation in human peripheral blood lymphocytes and murine T cells [ 33 63 ] . These reported differences are likely due to dose-dependent or cell-specific differences in the effect of SN50 [ 64 ] . Given that: (1) embryonic SMGs were cultured in the presence of 100 μg/ml SN50, (2) immunodetectable NF-κB was absent from SMG epithelia nuclei in TNF + SN50-treated explants, and (3) one cannot extrapolate observations in Jurkat cells to those in primary cells [ 64 ] or organ cultures, it is most probable that our observed interruption of SMG development is proximately due exclusively to the inhibition of NF-κB nuclear translocation.
Once optimal experimental conditions were established for each antibody, we then incubated blots of E15 and E17 SMGs in a mixture of these 4 antibodies and determined that we could identify all proteins in a single sample by M r . This methodology using a mixture of antibodies has been successfully used by Cell Signaling Technology and BD Signal Transduction for 2-D and 1-D Western blot analyses.
E15 SMGs were cultured for 4 days or longer in 10 U/ml recombinant mouse TNF (rTNF, R & D, Minneapolis, MN), 100 μg/ml SN50 + 10 U/ml rTNF, or 100 μg/ml mSN50 + 10 U/ml rTNF, 6-10 explants per treatment group.
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