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Example sentences for: smcs

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Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method was used to demonstrate apoptosis in SMCs and endothelial cells [ 6 ] . The TUNEL was performed on frozen sections.
One possible explanation for this apparent discrepancy is that although ERK1/2 are often involved in mitogenesis, in SMCs ERK activation can lead to inhibition of proliferation [ 46 ] . This occurs through ERK-mediated stimulation of PGE 2 production, which then inhibits the proliferation of SMCs [ 46 ] . Further experiments will determine whether RASMCs produce PGE 2 after treatment with III1-C.
III1-C is a recombinant fibronectin fragment that encompasses most of the first type III repeat, but is missing the A and B β-strands of the repeat [ 2 ] . We had previously found that III1-C inhibited the growth of normal diploid smooth muscle cells (SMCs) in culture [ 4 ] . During these experiments we noticed that the presence of III1-C in the culture medium affected the morphology of cells at early times after plating.
In addition, an antibody against α-actin (Enzo Diagnostics, Inc, Farmingdale, NY) was used to confirm SMCs in the media.
The adhesion of SMCs to fibronectin is mediated through β1 integrins [ 1 26 ] . We confirmed these previous findings for our RASMCs, by showing a complete block of cell attachment to fibronectin by EDTA (an agent that blocks all integrins), anti-β1 blocking mAb, and RGD peptide, but not by the negative control RAD peptide (Fig.