Words similar to site-directed

Example sentences for: site-directed

How can you use “site-directed” in a sentence? Here are some example sentences to help you improve your vocabulary:

First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
The availability of full-length and TPR-deleted recombinant PfPP5 clones will now allow us to further investigate the exact nature of the PfPP5-Pfhsp90 complexes, and map the interacting PfPP5 residues through site-directed mutagenesis.
This information suggested that residue 39 of LcrG was an ideal candidate for site-directed mutagenesis to disrupt LcrG's secretion blocking activity.
2 is a promoter-trap plasmid containing a promoterless lacZ gene isolated from λgt11 in which the Eco RI site in the 3' end of the lacZ gene was removed by site-directed polymerase-chain-reaction (PCR) mutagenesis (personal communication R. Van Landingham).
To introduce mutations within or nearby the proposed Fnr recognition site at the dmsA promoter, site-directed mutagenesis was performed using the method of Kunkel [ 33 ] . The template for mutagenesis was m13mp19-100 which contained a 676 bp Bam HI fragment containing 587 bp of DNA upstream of the dmsA translational start site and the associated 89 bp of the dmsA coding region.