Words similar to site-directed
Example sentences for: site-directed
How can you use “site-directed” in a sentence? Here are some example sentences to help you improve your vocabulary:
To introduce mutations within or nearby the proposed Fnr recognition site at the dmsA promoter, site-directed mutagenesis was performed using the method of Kunkel [ 33 ] . The template for mutagenesis was m13mp19-100 which contained a 676 bp Bam HI fragment containing 587 bp of DNA upstream of the dmsA translational start site and the associated 89 bp of the dmsA coding region.
The region from W89 through Y93 is shown as a β-sheet as has been hypothesized based on site-directed mutagenesis studies of this strand of the 5-HT 3 R [ 24 ] . Our data indicate the functional groups of lerisetron that may interact with W89, R91, Y142 and Y152.
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.
All site-directed mutagenesis were performed using the megaprimer procedure [ 51 ] , and the mutations were confirmed by sequencing.
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