Words similar to site-directed

Example sentences for: site-directed

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The site-directed mutagenesis of this glutamate severely retards the first and second electron transfers from the primary quinone that successively reduce the secondary quinone to semi-quinone and quinol [ 16].
Site-directed mutagenesis
These 'radical' site-directed mutants were then screened using the yeast two-hybrid system for their interaction with LcrV.
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
Methodology for the site-directed mutagenesis of pR is discussed in detail elsewhere (R.