Words similar to site-directed
Example sentences for: site-directed
How can you use “site-directed” in a sentence? Here are some example sentences to help you improve your vocabulary:
These obstacles have been largely circumvented by the use of cloned viral cDNA that is then altered by standard DNA-based site-directed mutagenesis procedures [ 8 9 ] . Originally designed for influenza virus minigenomes [ 10 ] , such cDNA-based "reverse genetics" strategy has been adopted in a large number of NNR viruses, including vesicular stomatitis virus (VSV), respiratory syncytial virus (RSV), and measles, to name a few [ 11 12 13 14 ] . Recently, extension of this approach has resulted in the cloning of full-length viral cDNA capable of producing infectious recombinant virus particles upon transcription.
Although members of the Ras GTPase superfamily have been studied for many years using site-directed mutagenesis approaches, there have been no previous reports demonstrating cytopathic effects of this kind.
These 'radical' site-directed mutants were then screened using the yeast two-hybrid system for their interaction with LcrV.
This generates the necessary 3' hydroxyl group for DNA polymerases (pol β, δ or ε, in eukaryotes) to insert the correct nucleotide in later steps in the base excision repair pathway (BER-pathway) [ 7 8 ] . Recent crystal structures of huAPE1 complexed with DNA containing an abasic site [ 9 10 11 ] , combined with sequence analysis and site-directed mutagenesis, have defined the residues that participate in metal ion based cleavage of the phosphate backbone of the DNA [ 12 13 14 15 16 17 ] .
proposed a reaction mechanism for the enzyme [ 37 ] . To test this proposed mechanism and the proposed roles of the amino acids discussed above, site-directed mutagenesis studies were conducted on metallo-β-lactamase L1 and reported herein.
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