Words similar to site-directed

Example sentences for: site-directed

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proposed a reaction mechanism for the enzyme [ 37 ] . To test this proposed mechanism and the proposed roles of the amino acids discussed above, site-directed mutagenesis studies were conducted on metallo-β-lactamase L1 and reported herein.
Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).
Site-directed mutagenesis of FtsZ
First, PfPP5 contained the catalytic core found in all Ser/Thr phosphatases of the PPP family, including the signature motifs such as GDXHGQ, GDFVDRG, RGNHE, HGLL, and SAPNYCD, to name a few [ 22 23 ] . Site-directed mutagenesis and structural studies in PP 1 and PPλ have previously established the roles of specific amino acid residues in these domains in the various aspects of catalysis, such as metal ion binding, phosphate recognition, and co-ordination of water molecules [ 24 25 26 ] .
To investigate the effects of sequence changes in the dmsA Fnr-recognition site on the anaerobic activation of dmsA-lacZ expression, site-directed mutagenesis and β-galactosidase assays were performed (Figure 1).