Words similar to rt-
Example sentences for: rt-
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We asked the following questions: 1) are the immuno-labeled structures within the nucleus or just closely associated; 2) is the phosphoepitope labeled by SMI-31 and RT-97 mABs specific to NFs or can it be identified on other IFs in other cell types; and 3) is there a relationship between the cell cycle as determined by DNA synthesis and the amount of nuclear labeling by SMI-31 and RT-97?
Therefore it is possible that this sequence constitutes or contains the epitope recognized by the SMI-31 and RT-97 mABs, and may account for the cross-reactivity of these antibodies with respect to NF-H, GFAP, and lamins.
Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
Comparison of nuclear labeling of SH-SY5Y cells immuno-stained with SMI-31 and RT-97 mABs to the culture proliferation index shows that nuclear labeling reached its greatest extent when DNA synthesis was also maximal.
In addition, Shea et al . [ 19 ] , in a study of neurite outgrowth from NB2a cells, showed that both the SMI-31 and RT-97 mABs strongly labeled nuclei of undifferentiated cells, and the nuclear labeling declined as differentiation progressed.
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