Words similar to rtta
Example sentences for: rtta
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For the 110 lines tested with rtTA(3)E2 there was an average 1.8% increase in the presence of DOX (Figure 2a), and for the 34 lines tested with rtTA(3)M1 an average 3.0% increase in the presence of DOX (Figure 2b), consistent with previous results that DOX itself has little to no effect on life span.
As a control, Oregon-R wild-type flies were repeatedly crossed to the rtTA strains and assayed for life span in parallel.
Each of the 144 new PdL insertion lines was crossed to the appropriate rtTA chromosome strain, and males containing both constructs were assayed in large cohorts (around 400 flies) for doxycycline-dependent effects on life span (Figure 2).
In an attempt to increase the variety of mutations identified, two different rtTA insertion strains were used.
When flies are fed doxycycline, rtTA binds to target sequences in the synthetic tet-on promoter in the PdL P element.