Words similar to rt-pcr

Example sentences for: rt-pcr

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As reported previously, the long RT-PCR method identified six samples with transcripts skipping exons 18, 31, 46, 49, 56 or 58 that were caused by nucleotide substitutions at splice sites [ 8 ] . In another patient, skipping of exon 51 was discovered to be due to a silent mutation within the exon, possibly affecting a splicing enhancer binding site [ 19 ] . Here we report abnormal fragments seen after Taq I digestion of the exon 29-52 amplicons (Fig.
The levels of MECP2 transcripts were virtually identical by real-time quantitative RT-PCR analysis (less than 0.27 cycle thresholds difference) across all eight clones, one wild-type and one mutant from each individual, that were used for oligonucleotide array hybridization (data not shown).
In all LM epididymal samples from humans (Subjects 1 and 3 corpus, Subject 2 caput/corpus) and macaques (#1, corpus and 2, cauda) the expected 320 bp SPAM1 cDNA fragment was amplified in the RT-PCR reactions.
Expression of the ribozyme targeted against the M6P/IGF2 receptor in MCF-7 cells resulted in down-regulation of M6P/IGF2R expression, as measured by RT-PCR, and M6P/IGF2R function, indicated by a decrease in internalization of 125I-IGF-II, and β-glucuronidase binding and endocytosis.
Reverse transcription of RNA was performed using the Superscript II first strand synthesis system for RT-PCR (Gibco BRL), following the supplier's instructions.