Example sentences for: rt-pcr

How can you use “rt-pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • IgM - immunoglobulin of the M isotype; ova - chicken ovalbumin; PY - phosphotyrosine; PLCγ2 - phospholipase C γ2; anti - antibody or antiserum generated against indicated antigen; RT-PCR - reverse transcriptase polymerase chain reaction; ICKA - immune-complex kinase assay; ITAM - immunoreceptor tyrosine-based activation motifs; SH2 and SH3 - Src homology domains 2 and 3; PBS - phosphate-buffered saline.

  • Whether there is compensation by increased expression of other γ subunits such as the γ 6 and γ 7 subunits, which were shown recently to be present in skeletal muscle by RT-PCR [ 19], or alteration of the amount of the other subunits remains to be determined in the future.

  • A semi-quantitative analysis of gene expression was performed in replicate experiments using 26-28 cycles of multiplex RT-PCR with β-actin ( ACTB ) as control and gene specific primers spanning at least 2 exons.

  • Quantitative RT-PCR was performed on four genes (three with increased and one with decreased expression) to assess the accuracy of GeneChip results.

  • It is possible that the UV irradiation damaged other regions of the viral RNA and not the region that our primers amplified, or that the amount of damage required to prevent RT-PCR amplification is greater than the amount of damage sufficient to prevent infection.


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