Words similar to rt-pcr

Example sentences for: rt-pcr

How can you use “rt-pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

In earlier reports, Northern blot hybridization and RT-PCR failed to detect DFz1, DFz2, and DFz3 in S2 cells [ 6 17 18 ] . Using a one-step RT-PCR assay to measure the expression levels of all 4 DFzs and Arr in the same S2 cells used by Yanagawa et al . (Figure 1A), we detected DFz2, DFz3, and Arr mRNAs, but not DFz1 and DFz4 mRNAs after 40 cycles of amplification.
Here we report quantification of the effects of CDDO on gene expression in the human chondrosarcoma cell line SW-1353 with the use of real-time reverse-transcriptase polymerase chain reaction (RT-PCR).
To further investigate this possibility, we performed RT-PCR to generate cDNAs that spanned exon 20 of EGFR and included sequences from exon 19 and 21.
RT-PCR followed by sequencing was also employed to confirm the predicted translation start sites.
Semi-quantitative RT-PCR using specific primers for TNF-α, MIP-2 (CXCL2) and IL-1β showed that LPS treatment caused the amount of TNF-α mRNA to slightly increase compared to the already abundant levels found in untreated cells (figure 2and 3).