Example sentences for: rt-pcr

How can you use “rt-pcr” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • JY performed RT-PCR, northern and DNA sequencing analysis.

  • RNA and protein samples were analyzed as follows: Podocyte RNA (10 μg) was analyzed by northern blot using probes for HIV-1 nef-LTR [ 7 ] , mouse cyclin D 1 (cDNA generated by RT-PCR from mouse podocytes and verified by sequence analysis), synaptopodin [ 7 ] , and G3PDH.

  • For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).

  • APC = allophycocyanin; ARE = AU-rich element; CM = complete media; ELISA = enzyme-linked immunosorbent assay; FACS = fluorescence-activated cell sorting; FITC = fluorescein isothiocyanate; IL = interleukin; KLH = keyhole limpet hemocyanin; LPS = lipopolysaccharide; MESF = milliequivalents of soluble fluorescein; RPMI = Roswell Park Memorial Institute [medium]; RT-PCR = reverse transcriptase polymerase chain reaction; TNF = tumor necrosis factor; TNFR = TNF receptor; TTP = tristetraprolin; WBC = white blood cell.

  • The relative amounts of Abcg5 , Abcg8 , Cyp7a1, HMG-CoA reductase, and Srebp-2 message from rat liver samples used in real-time RT-PCR were normalised to GAPDH expression levels to compensate for variations in input RNA amounts.


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