Example sentences for: reactions

How can you use “reactions” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA was reverse transcribed using Superscript II RNase H -Reverse Transcriptase (Life Technologies, Inc.) as per manufacturer's protocol, except that all reactions were doubled to 40 μL final volume.

  • Coupling reactions were quenched by the addition of 41 μl of 0.1 M sodium acetate pH 5.2, and unincorporated dye was removed using QIAquick columns.

  • The method was adapted from the procedure published by the Dixon lab [ 15 16 ] . PCR reactions were performed using primers containing the T7 polymerase binding site.

  • PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).

  • All reactions were incubated at 30°C for 20 min.


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