Words similar to rasmcs
Example sentences for: rasmcs
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One way in which integrins were originally shown to be fibronectin receptors was by affinity chromatography of radioiodinated cell surface proteins on a fibronectin column [ 27 28 ] . Consistent with these earlier studies, cell surface radioiodination of RASMCs followed by affinity chromatography on fibronectin Sepharose and elution with EDTA revealed the binding of two major bands at 120 kDa and 140 kDa (Fig.
To identify the cell surface proteoglycans that may serve as receptors for III1-C, RASMCs were labeled with 35SO 4 and cell lysates were applied to III1-C and III 11-C columns.
Primary rat aortic smooth muscle cells (RASMCs) were isolated from 12-16 week old Sprague-Dawley rats as described previously [ 51 ] . RASMCs were cultured in DMEM supplemented with 10% FBS and glutamine Pen-Strep.
The attachment of RASMCs to III1-C was analyzed further to determine the role played by integrins as potential adhesive receptors for III1-C.
Growing RASMCs were harvested by trypsinization and collected into DMEM + 0.5% BSA + soybean trypsin inhibitor.
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