Words similar to rasmcs
Example sentences for: rasmcs
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Primary rat aortic smooth muscle cells (RASMCs) were isolated from 12-16 week old Sprague-Dawley rats as described previously [ 51 ] . RASMCs were cultured in DMEM supplemented with 10% FBS and glutamine Pen-Strep.
These results indicate that most of the cell surface HSPGs of RASMCs can bind to III1-C in affinity chromatography.
The adhesion of SMCs to fibronectin is mediated through β1 integrins [ 1 26 ] . We confirmed these previous findings for our RASMCs, by showing a complete block of cell attachment to fibronectin by EDTA (an agent that blocks all integrins), anti-β1 blocking mAb, and RGD peptide, but not by the negative control RAD peptide (Fig.
One way in which integrins were originally shown to be fibronectin receptors was by affinity chromatography of radioiodinated cell surface proteins on a fibronectin column [ 27 28 ] . Consistent with these earlier studies, cell surface radioiodination of RASMCs followed by affinity chromatography on fibronectin Sepharose and elution with EDTA revealed the binding of two major bands at 120 kDa and 140 kDa (Fig.
Growing RASMCs were harvested by trypsinization and collected into DMEM + 0.5% BSA + soybean trypsin inhibitor.