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Example sentences for: rassf

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We found that azacytidine treatment resulted in reactivation of gene expression in almost all cell lines that showed promoter methylation of MGMT, RASSF1A and RARB genes, with the exception of the cell line Tera-1.
4%) have also reported a similar overall high frequency of promoter methylation [ 12 13 ] . We identified promoter methylation in >25% cases in CDH1 , DAPK , and RARB genes, while it was less frequent (10-25% cases) in HIC1 , FHIT , RASSF1A , and APC genes in CC patients.
Promoter methylation was frequently seen in DNA repair genes MGMT , RASSF1A , and BRCA1 , and a transcriptional repressor gene HIC1 . Promoter methylation of most genes resulted in transcriptional repression.
The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
Azacytidine treatment reactivated expression of RASSF1A in one of each of the two methylated and two unmethylated cell lines.