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Example sentences for: rassf

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Levels of expression of each gene were assessed by comparing with the respective control values, obtained from the averages calculated from 2 to 4 normal testes, after normalization against ACTB . All tumors with promoter hypermethylation of the MGMT and MLH1 genes exhibited an absence or down-regulated expression of the respective gene, while 8 of 10 cases with RASSF1A methylation and 3 of 5 tumors with RARB methylation showed down-regulated expression (Table 1).
The primers used for CDH1 , MGMT , MLH1 , and RARB have been described earlier [ 50 ] . The gene primers used for DAPK and RASSF1A and their positions in respective cDNAs were:
RASSF1A -MF 5'-ACGCGTTGCGTATCGCGCG-3'
Although the patterns are similar, primary tumors had a higher frequency of methylation of CDH1 and DAPK genes as compared to cell lines, while RARB , HIC1 , RASSF1A , MGMT , and TP73 genes had higher prevalence in cell lines as compared to primary tumors (Table 1).
RASSF1A -UR 5'-CCACAACAACTACACTACCCC-3'