Example sentences for: pvl

How can you use “pvl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pBS-KS +-NFATp was generated by digesting pREP-NFATp with BssH II and Dra I, filling in the ends with Klenow, and ligating the resulting fragment into the Sma I site of pBS-KS +. pVL1392-HAX-NFATp was generated by subcloning a fragment excised from pBS-KS +-NFATp with Spe I and EcoR I into pVL1392-HAX (gift of S. Ruppert and R. Tjian) digested with Spe I and EcoR I. pBS-KS +-NFATp(NdeI), which has an Nde I site, containing the NFATp translational start site ATG, was generated in two steps.

  • The PAI-1 sequence containing the desired mutations was digested with EcoR1 and BglII, and then subcloned into pVL1393.

  • A pVL1392-HAX-NFATp(331-722) was made by subcloning a Nde I - Sma I fragment from pGEX-NFATp(331-722) (J.

  • Goodrich, unpublished) into the Nde I and Sma I sites of pVL1392-HAX.

  • In the second step, the Nde I EcoR I fragment from pBS-NFATp(391-583) was ligated into the Nde I and EcoR I sites of pVL1392-GST.


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