Example sentences for: pvl

How can you use “pvl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pVL1393-HAX-NFATp(331-921) was made by digesting pBS-KS +-NFATp with SgrA I and filling in the overhang by treatment with Klenow.

  • The PCR product was digested with Xba I and Mro I and ligated into pBS-KS +-NFATp that had been digested with Xba I and Mro I. pVL1392-HAX-NFATp(1-722) was made by subcloning a Nde I - Msc I fragment from pBS-KS +-NFATp(NdeI) into the Nde I and Sma I sites of pVL1392-HAX.

  • The resultant pVL1393-PAI-1 was analyzed by restriction mapping and sequencing.

  • The PAI-1 sequence containing the desired mutations was digested with EcoR1 and BglII, and then subcloned into pVL1393.

  • In the second step, the Nde I EcoR I fragment from pBS-NFATp(391-583) was ligated into the Nde I and EcoR I sites of pVL1392-GST.


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