Words similar to pvdf

Example sentences for: pvdf

How can you use “pvdf” in a sentence? Here are some example sentences to help you improve your vocabulary:

Samples containing equivalent amounts of protein were resolved by 12% polyacrylamide SDS PAGE under reducing conditions and then electroblotted onto PVDF membranes.
The proteins were denatured by boiling for 5 min, then were separated by electrophoresis on 7.5% SDS-PAGE, and transferred to PVDF membranes.
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
Separated proteins were electroblotted to PVDF membranes according to manufacturer's recommendations (Roche Molecular Biochemicals).
Due to the high molecular weight of PARP, SDS-PAGE was performed using 7% polyacrylamide gels, and proteins were then transferred to PVDF membrane (Biorad) via wet transfer.