Example sentences for: pvdf
How can you use “pvdf” in a sentence? Here are some example sentences to help you improve your vocabulary:
Proteolytic fragments separated by SDS-PAGE were transferred to ProBlott PVDF membranes (Applied Biosystems, Inc.) as described by LeGendre et al [ 38 ] . Coomassie-stained protein bands were then subjected to sequence analysis using either a Model 491 or Model 470A gas phase sequencer equipped with a Model 120A on-line phenylthiohydantoin analyzer (Applied Biosystems, Inc).
GAG detection was also performed by the Toluidine Blue-O precipitation and dot-blot method as described previously [ 34 ] . Briefly, 200 μl of sample were applied to each well of a 48-well manifold (Life Technologies) onto a PVDF membrane without vacuum.
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.
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