Words similar to pvdf

Example sentences for: pvdf

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Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
Samples were transferred to PVDF, immunoblotted versus AB129, and developed using ECL Plus.
AID (α subunit interaction domain); BID (β subunit interaction domain); DMSO (dimethyl sulfoxide); EGTA (ethylene glycol bis-aminoethylether tetraacetic acid); HEPES (2-hydroxylethyl piperazine 2-ethane sulfonic acid); MOPS (3N-Morpholino-propane sulfonic acid); PVDF (polyvinylidene difluoride); TTX (tetrodotoxin); TEA (tetraethylamonium).
Due to the high molecular weight of PARP, SDS-PAGE was performed using 7% polyacrylamide gels, and proteins were then transferred to PVDF membrane (Biorad) via wet transfer.
Nitro blue tetrazolium (NBT), 5-bromo-4-chloroindol-3-yl phosphate (BCIP) and poly(vinylidene difluoride) (PVDF) were from Bio-Rad.