Example sentences for: pvdf
How can you use “pvdf” in a sentence? Here are some example sentences to help you improve your vocabulary:
PIP3: Phosphatidyl-3,4,5-triphosphate; DMEM: Dulbecco's Modified Eagle's Medium; PI-3Kinase: phosphatidylinositol-3-kinase; MAPK: mitogen-activated protein kinase; IGF: insulin-like growth factor; PTEN: phosphatase and tensin homolog deleted from chromosome 10; SOS: son-of-sevenless ras exchange factor; ASK: apoptosis stimulating kinase; FKHR: forkhead related protein; cFLIP: cellular FLICE interacting protein; BSA: bovine serum albumin; TBS: tris buffered saline; PVDF: polyvinylidene difluoride
Samples were transferred to PVDF, immunoblotted versus AB129, and developed using ECL Plus.
Proteolytic fragments separated by SDS-PAGE were transferred to ProBlott PVDF membranes (Applied Biosystems, Inc.) as described by LeGendre et al [ 38 ] . Coomassie-stained protein bands were then subjected to sequence analysis using either a Model 491 or Model 470A gas phase sequencer equipped with a Model 120A on-line phenylthiohydantoin analyzer (Applied Biosystems, Inc).
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
GAG detection was also performed by the Toluidine Blue-O precipitation and dot-blot method as described previously [ 34 ] . Briefly, 200 μl of sample were applied to each well of a 48-well manifold (Life Technologies) onto a PVDF membrane without vacuum.
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