Example sentences for: pvdf
How can you use “pvdf” in a sentence? Here are some example sentences to help you improve your vocabulary:
After blotting, the PVDF membrane was stained with 2% amido black in 7% glacial acetic acid and the protein fronts of all lanes were compared for loading accuracy.
Separated proteins were electroblotted to PVDF membranes according to manufacturer's recommendations (Roche Molecular Biochemicals).
Proteins were electroblotted to Hybond-P PVDF membranes (Amersham Pharmacia Biotech), blocked with gelatin, and probed with affinity purified rabbit anti-human myocilin antibody 129 (AB129; generated to human myocilin amino acids 156-171) [ 12 17 ] and an anti-rabbit IgG secondary antibody (Amersham).
One hundred thousand cells were separated by 5% SDS-PAGE, transferred by wet-transfer to PVDF membrane, and blotted as described above using monoclonal antibody specific for the N-terminus of BRCA1 (Oncogene Boston, MA).
Samples containing equivalent amounts of protein were resolved by 12% polyacrylamide SDS PAGE under reducing conditions and then electroblotted onto PVDF membranes.
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