Words similar to pvdf

Example sentences for: pvdf

How can you use “pvdf” in a sentence? Here are some example sentences to help you improve your vocabulary:

Proteins were electroblotted to Hybond-P PVDF membranes (Amersham Pharmacia Biotech), blocked with gelatin, and probed with affinity purified rabbit anti-human myocilin antibody 129 (AB129; generated to human myocilin amino acids 156-171) [ 12 17 ] and an anti-rabbit IgG secondary antibody (Amersham).
The proteins were transferred to a PVDF transfer membrane (BIO-RAD, Hercules, CA, USA) in electrophoresis buffer (20 mM Tris-HCl, pH8.
After blotting, the PVDF membrane was stained with 2% amido black in 7% glacial acetic acid and the protein fronts of all lanes were compared for loading accuracy.
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Separated proteins were electroblotted to PVDF membranes according to manufacturer's recommendations (Roche Molecular Biochemicals).