Words similar to puc
Example sentences for: puc
How can you use “puc” in a sentence? Here are some example sentences to help you improve your vocabulary:
After confirmation of the sequence, the mutated pL1PUC19 plasmid was digested with Nde I and Hind III, and the 900 bp, mutated L1 gene was gel purified and ligated into pET26b to create the mutant overexpression plasmids.
[ 60 ] , as described previously [ 68 ] . The oligonucleotides used for the preparation of the mutants are shown in Table 1. The ca. 900 bp PCR products were digested with Nde I and Hind III and ligated into pUC19.
pCL8 is a derivative of pCL5 [ 9 ] and contains the luciferase ( luc ) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene ( aph ) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the P
The vector pUC19 (Yanisch-Perron et al.
Additional restriction mapping studies suggest that the hypersensitive regions are within the viral DNA fragment, and not in pUC DNA (data not shown).