Example sentences for: puc

How can you use “puc” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.

  • The vector pUC19 (Yanisch-Perron et al.

  • The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.

  • [ 60 ] , as described previously [ 68 ] . The oligonucleotides used for the preparation of the mutants are shown in Table 1. The ca. 900 bp PCR products were digested with Nde I and Hind III and ligated into pUC19.

  • Nick-translated biotin-labeled pUC18 plasmid was used as a control for non-specific probe binding and HPV probes were also applied to normal cervical tissues.


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