Example sentences for: puc

How can you use “puc” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The plasmid pTK2 contains HSV-2 SB5 sequence coordinates 44,469-48,184 subcloned into the HindIII / EcoRI site of pUC19.

  • K562 cells expressing integrin α4, α3, or α3 integrin subunit lacking a cytoplasmic tail (K562-X3C0) were described elsewhere [ 30 40 ] . Also utilized were K562 cells expressing α3 D154A, W220A and Y218A mutants [ 41 ] , and Chinese hamster ovary (CHO) cells that express human α2, α3, α5 or β1 integrin subunits [ 30 32 ] . The cDNA encoding human CD98 heavy chain [ 57 ] was provided by Jeffrey Leiden (University of Chicago) in the pUC18 vector.

  • Plasmids pET26b and pUC19 were purchased from Novagen.

  • 1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.

  • pCL8 is a derivative of pCL5 [ 9 ] and contains the luciferase ( luc ) gene from pT3/T7LUC (Clontech, Palo Alto, CA), the mycobacteriophage L5 int gene, the kanamycin-resistance gene ( aph ) from pUC4-KIXX (Amersham Biosciences, Piscataway, NJ), and the P


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