Words similar to puc
Example sentences for: puc
How can you use “puc” in a sentence? Here are some example sentences to help you improve your vocabulary:
The plasmid pLacZ contains the LacZ coding region from pSVβgal subcloned into the HindIII / EcoRI site of pUC19.
Figures 4and 7demonstrate normal cervix and CIN I tissues hybridized with biotin-labeled pUC18 vector.
The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.
1) A promoterless TN5 aph gene was PCR amplified from pUC4-KIXX as an 850-bp fragment using primers 1915 and 1916, and a promoterless xylE gene was PCR amplified from pTKMX97 as a 935-bp fragment using primers 1917 and 1918.
After polishing the ends of the fragments using Klenow (New England Biolabs, Beverly, MA), they were cloned into SmaI -restricted/CIAP pUC18 vector.
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