Words similar to puc

Example sentences for: puc

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Plasmids pET26b and pUC19 were purchased from Novagen.
[ 60 ] , as described previously [ 68 ] . The oligonucleotides used for the preparation of the mutants are shown in Table 1. The ca. 900 bp PCR products were digested with Nde I and Hind III and ligated into pUC19.
After confirmation of the sequence, the mutated pL1PUC19 plasmid was digested with Nde I and Hind III, and the 900 bp, mutated L1 gene was gel purified and ligated into pET26b to create the mutant overexpression plasmids.
K562 cells expressing integrin α4, α3, or α3 integrin subunit lacking a cytoplasmic tail (K562-X3C0) were described elsewhere [ 30 40 ] . Also utilized were K562 cells expressing α3 D154A, W220A and Y218A mutants [ 41 ] , and Chinese hamster ovary (CHO) cells that express human α2, α3, α5 or β1 integrin subunits [ 30 32 ] . The cDNA encoding human CD98 heavy chain [ 57 ] was provided by Jeffrey Leiden (University of Chicago) in the pUC18 vector.
The amplified annealed fragment was gel purified and cloned into the Sma I site of pUC19 to generate pSE.