Words similar to puc

Example sentences for: puc

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A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (ori S ) SmaI fragment [ 5 ] . The plasmid pTK1 contains HSV-1 SC16 sequence coordinates 45,055-48,634 subcloned into the HindIII / EcoRI site of pUC19 (Invitrogen).
K562 cells expressing integrin α4, α3, or α3 integrin subunit lacking a cytoplasmic tail (K562-X3C0) were described elsewhere [ 30 40 ] . Also utilized were K562 cells expressing α3 D154A, W220A and Y218A mutants [ 41 ] , and Chinese hamster ovary (CHO) cells that express human α2, α3, α5 or β1 integrin subunits [ 30 32 ] . The cDNA encoding human CD98 heavy chain [ 57 ] was provided by Jeffrey Leiden (University of Chicago) in the pUC18 vector.
The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.
The amplified annealed fragment was gel purified and cloned into the Sma I site of pUC19 to generate pSE.
Figures 4and 7demonstrate normal cervix and CIN I tissues hybridized with biotin-labeled pUC18 vector.