Example sentences for: ptztd

How can you use “ptztd” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The constructs were made using standard solid phase DNA synthesis, then were PCR-amplified and cloned into pTZtd1304.

  • The PCR product was digested with SpeI (New England Biolabs, Beverly, MA; 20 units) and EcoRI (50 units) in buffer (50 mM NaCl, 100 mM Tris-HCl, pH 7.5, 10 mM MgCl 2 , 0.025% Triton X-100, 100 μg/ml BSA) at 37°C for 60 minutes, purified, and cloned into SpeI/EcoRI digested pTZtd1304.

  • The wild type plasmid pTZtd1304 [ 17 ] contains a fully functional 265 nt derivative of the 1016 nt wild type intron [ 15 ] . This derivative also contains the substitutions U34A, which introduces a SpeI site, and U976G, which introduces an EcoRI site.


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