Words similar to ptx-mkc

Example sentences for: ptx-mkc

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A FLAG epitope was fused to the amino-terminus of MHCK-C at codon 2 using the vector pTX-FLAG [ 36 ] . The resulting plasmid, pTX-MKC2, was transformed into the cell line Ax2 and clonal cell lines were selected with G418 (8 μg/ml) in HL-5 medium.
The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail [ 24 ] . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
pTX-MKA1, pMKB-GFP, pTX-MKC1 were transformed into either Ax2 cell line [ 37 ] or HS1 myosin null cells, a strain of D. discoideum with its endogenous mhc A gene deleted [ 38 ] to generate GFP-MHCK-A, -B, and -C cells, respectively.
To facilitate FLAG-MHCK-C protein purification, Ax2/pTX-MKC2 cell lines were then subjected to incremental increases in G418 selection level over approximately 3 weeks, to a final selection level of 40 μg/ml.
For purification of FLAG-MHCK-C, 8-10 liters of 3xALA/pTX-MKC2 cells were propagated in suspension culture in HL-5 medium to approximately 5 × 10 6cells/ml.