Words similar to prlg
Example sentences for: prlg
How can you use “prlg” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PrlA (SecY) and PrlG (SecE) suppressors are compromised in their ability to proofread, allowing export of proteins with mutations, or even complete deletions, of the signal sequence.
Although prlA and prlG strains export secretory proteins that completely lack a signal sequence, there is no evidence of export of any cytoplasmic proteins in a prl suppressor strain [ 13 ] . That is, proteins that are supposed to remain in the cytoplasm are not mislocalized to the periplasm.
The signal sequence is crucial for efficient translocation; mutations in the signal sequence significantly reduce export of the preprotein and complete deletions of the signal sequence eliminate essentially all export [ 5 6 7 8 ] . Selection for extragenic suppressors of such export defective preproteins led to the identification of the prl alleles of secY ( prlA ), secE ( prlG ), secA ( prlD ), and more recently secG ( prlH ) [ 6 9 10 11 ] . Early hypotheses predicted that the Prl suppressors function by expanded or altered interactions with signal sequences, facilitating recognition of mutant as well as wild type signal sequences [ 6 ] .
In fact, all of the PrlA and PrlG suppressors are completely dependent on functional SecB for manifestation of suppressor activity [ 5 8 ] . Even certain proteins that are not normally SecB-dependent for translocation become SecB-dependent when they contain a signal sequence mutation and are exported via the suppressor pathway.
In a wild type cell, the mutant precursor would be rejected at this point by SecY/E, but in a PrlA or PrlG suppressor strain, rejection would not occur and the mutant secretory protein would be exported.
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