Words similar to prl
Example sentences for: prl
How can you use “prl” in a sentence? Here are some example sentences to help you improve your vocabulary:
There were two reasonable explanations for this unexpected finding; 1) an unfolded state is not sufficient for SecB binding, or 2) SecB binding is not sufficient to promote export in a prl suppressor strain.
Although prlA and prlG strains export secretory proteins that completely lack a signal sequence, there is no evidence of export of any cytoplasmic proteins in a prl suppressor strain [ 13 ] . That is, proteins that are supposed to remain in the cytoplasm are not mislocalized to the periplasm.
In a Prl suppressor strain, however, the proofreading function would be compromised and the mutant protein would be exported [ 8 ] . In the experiments described here, we tested this hypothesis by examining the export of such a cytoplasmic protein with a folding mutation.
It has been suggested that perhaps cytoplasmic proteins are not exported in prl suppressor strains because they fold rapidly and escape recognition by SecB, while secretory proteins, even those lacking a signal sequence, fold more slowly, allowing time for SecB recognition and binding [ 5 13 ] . SecB binds to a variety of unfolded proteins in vitro [ 24 25 ] , although binding appears to be more selective in vivo [ 26 ] . This selectivity may be based on the slower folding characteristics of secretory proteins.
The signal sequence is crucial for efficient translocation; mutations in the signal sequence significantly reduce export of the preprotein and complete deletions of the signal sequence eliminate essentially all export [ 5 6 7 8 ] . Selection for extragenic suppressors of such export defective preproteins led to the identification of the prl alleles of secY ( prlA ), secE ( prlG ), secA ( prlD ), and more recently secG ( prlH ) [ 6 9 10 11 ] . Early hypotheses predicted that the Prl suppressors function by expanded or altered interactions with signal sequences, facilitating recognition of mutant as well as wild type signal sequences [ 6 ] .
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