Words similar to prla
Example sentences for: prla
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In the secY wild type strain, proofreading by SecE/Y would block export of LA57-N 102LT, while in the prlA4 strain, the PrlA4 suppressor would allow export of a portion of LA57-N 102LTto the periplasm.
Plasmids pKL106 and pKL107 encoding LA57-N 102LT-FLAG or N 102LT-FLAG, respectively, were transformed into strains IM104 (wild type secY ) or IM105 ( prlA4 ). These strains contained deletion mutations in the lon and degP genes to limit proteolysis in both the cytoplasmic and periplasmic spaces.
The signal sequence is crucial for efficient translocation; mutations in the signal sequence significantly reduce export of the preprotein and complete deletions of the signal sequence eliminate essentially all export [ 5 6 7 8 ] . Selection for extragenic suppressors of such export defective preproteins led to the identification of the prl alleles of secY ( prlA ), secE ( prlG ), secA ( prlD ), and more recently secG ( prlH ) [ 6 9 10 11 ] . Early hypotheses predicted that the Prl suppressors function by expanded or altered interactions with signal sequences, facilitating recognition of mutant as well as wild type signal sequences [ 6 ] .
Therefore, there was no detectable export of LA57-N 102LTin the prlA4 strain.
All bacterial strains used were derivatives of E. coli K12 strain MC4100 [ 29 ] . IM104 is MC4100 lamB14D lon ::Tn 10 degP :: kan and IM105 is IM104 prlA4 . Plasmids pKL106 and pKL107 are pET11c (Novagen) derivatives carrying tandem copies of the λ repressor fragment (derived from plasmids pAD103 and pAD105, generous gifts from Alan Davidson, University of Toronto and Robert Sauer, MIT).
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