Words similar to ppt
Example sentences for: ppt
How can you use “ppt” in a sentence? Here are some example sentences to help you improve your vocabulary:
Using the program Modeller [ 26 ] , a homology model of the catalytic domain of Ppt1p was built based on three PP1 structures - PDB http://www.pdb.org [ 27 ] codes 1FJM [ 28 ] , 1IT6 [ 29 ] and 1JK7 [ 30 ] . This model shows that the catalytic face of Ppt1p is generally less acidic than that of PP1c γ and does not indicate the presence of other acidic residues that could act in the same fashion as those on PP1c γ.
Budding yeast lacking PPT1 do not exhibit obvious growth defects or detectable phenotypes [ 2 ] . Disruption of PPT1 together with one of the other known phosphatases in budding yeast also reveals no obvious phenotype [ 23 ] . Thus, if Ppt1p has an essential function in S. cerevisiae , more than one other phosphatase can also carry out this role.
The pattern of PPT1 gene expression was identical in wild-type cells and cells expressing HA-Ppt1p, indicating that the epitope tag did not alter transcription or message stability (data not shown).
The crystal structure of the PP5 TPR domain revealed three TPRs followed by an extended α helix [ 24 ] , however Ppt1p was originally proposed to contain one additional TPR [ 2 ] . The pattern of proteolysis and sites of cleavage for Ppt1p are very similar to those seen with PP5 under these same conditions [ 19 ] . This indicates that Ppt1p also contains an accessible region linking the TPR and catalytic domains and is most consistent with the presence of three TPRs in Ppt1p.
Removal of the TPR domain from Ppt1p by trypsin treatment had no effect on its activity toward 32P-casein, but increased activity toward 32P-MBP approximately two fold (Fig.
Loading...