Example sentences for: post-transfection

How can you use “post-transfection” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were switched to serum-free media 24 h post-transfection and media or cell lysates were collected after overnight incubation.

  • Metabolic labeling of cells and immunoprecipitation was performed essentially as described previously [ 21 30 ] . In brief, confluent monolayers of A549 cells were transfected with appropriate dsRNA (at 20 nM final) as described above, and at 8 hr post-transfection, were washed with a phosphate-free buffer (120 mM NaCl, 5 mM KCl, 1.5 mM MgCl 2 , 0.25 mM CaCl 2 , 25 mM NaHCO 3 , 20 mM HEPES [pH 7.4]) and labeled with 32P-orthophosphate (500 μCi/ml) (Amersham) in Dulbecco-modified Eagle MEM lacking both phosphate and pyruvate.

  • The cell medium was replaced 24 h post-transfection with medium containing G418 (400 μg/ml) or hygromycin (500 μg/ml) to select for stable transfectants.

  • GFP-Hyal-2 enhanced WOX1-mediated L929 cell death, as determined 48 hr post-transfection using crystal violet staining (Fig.

  • LMH cells were transfected by calcium phosphate coprecipitation as described previously [ 6], and viral cores were isolated 3 days post-transfection.


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