Words similar to peptide
Example sentences for: peptide
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The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
Design of the Primary Peptide Libraries
Immunohistochemical staining was done using rabbit polyclonal antibodies raised against synthetic peptides that correspond to regions in the mature forms of TGF-β 1 , TGF-β 2 , and TGF-β 3 . Antibodies to TGF-β 1 were raised against a synthetic peptide corresponding to residues 1-30 of the mature protein in either the Laboratory of Chemoprevention (anti-TGF-β 1 -LC; NIH, Bethesda, MD, USA) or the Collagen Corporation (anti-TGF-β 1 -CC; Palo Alto, CA, USA).
Briefly, cells were stained with anti-human CD8− fluorescein isothiocyanate (Caltag Laboratories, Burlingame, California, United States) and CD19-CyChrome (BD Biosciences, Palo Alto, California, United States) Abs, and HLA-A*0201/peptide tetramer–phycoerythrin (PE).
Heteroclitic peptide analogs are created by substitutions at anchor residues resulting in increased association of peptide with the major histocompatibility complex (MHC) [10].