Words similar to pepstatin

Example sentences for: pepstatin

How can you use “pepstatin” in a sentence? Here are some example sentences to help you improve your vocabulary:

Oocytes were prepared after the method of Rho et al . [ 29 ] . Typically 30 oocytes were lysed by trituration in 200 μL of a solution containing 100 mM Tris-HCl, 100 mM NaCl, 0.5% Triton X-100, 0.05 mg/mL pepstatin, 0.05 mg/mL leupeptin, and 0.05 mg/mL aprotinin (pH 8.0) and the homogenate was incubated for 15 minutes on ice.
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
Protein A, the protease inhibitors phenylmethyl-sulfonyl fluoride, leupeptin and pepstatin, and 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) were all from Sigma, St. Louis, MO.
Cells were suspended in buffer containing 50 mM HEPES pH 7.4, 10 mM KCl, 1 mM EDTA, 1 mM DTT, 1 μg/ml pepstatin A, 1 μg/ml leupeptin, 10 μg/ml soybean trypsin inhibitor, 10 μg/ml aprotinin and 0.5% NP-40 and mixed periodically using a vortex.
4; 150 mM NaC1; 0.5% Triton X-100, 10 ug/ml leupeptin, 10 ug/ml pepstatin, 17 ug/ml aprotinin, 1 mM PMSF).