Words similar to pepstatin

Example sentences for: pepstatin

How can you use “pepstatin” in a sentence? Here are some example sentences to help you improve your vocabulary:

HA-NFATp was subsequently eluted with 1 mg/ml HA peptide resuspended in elution buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 5 mM MgCl 2 , 0.2 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).
Cell were harvested by low-speed centrifugation and resuspended in lysis buffer (20 mM Tris (pH 7.9), 20% (v/v) glycerol, 1 mM EDTA, 0.5 M NaCl, 0.1% NP-40, 0.2 mM PMSF, 1 μg/ml leupeptin, 1.4 μg/ml pepstatin A, and 1 mM DTT).
Next, the cells were resuspended at 3 × 10 6cells/ml in ice cold sonication buffer (250 mM sucrose; 10 mM HEPES, pH 7.4; 1 mM EDTA; 5 mM MgCl 2 ; 1 mM DTT; 0.1 mM GTP; 1 μg/ml leupeptin, 1 μg/ml pepstatin A, and 1 μg/ml aprotinin; 1 mM PMSF) and sonicated for 10 seconds.
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
They were then lysed in a protein kinase lysis buffer [50 mM HEPES, pH 7.5, 150 mM NaCI, 1.5 mM MgCI2, 1 mM EGTA, 1 mM sodium vanadate, 50 mM sodium fluoride, 20 mM beta-glycerophosphate, 10% (vol/vol) glycerol, 1% (vol/vol) Triton X-100, and protease inhibitors (1 mM PMSF, 1 ug/ml pepstatin A1, 1 ug/ml leupeptin, and 1 ug/ml aprotinin].