Words similar to pepstatin
Example sentences for: pepstatin
How can you use “pepstatin” in a sentence? Here are some example sentences to help you improve your vocabulary:
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.
Protein lysates from whole cells and tissues were prepared with a buffer, which contained 50 mM Tris, pH 7.5, 150 mM NaCl, 1% (v/v) Nonidet P40 (NP40), 2 mM 4-(2-aminoethyl)benzenesulphonyl fluoride, 1 μg/ml leupeptin, 2 μg/ml aprotinin and 2 μg/ml pepstatin.
The Triton-insoluble fractions were then scraped into cold Triton X-100 lysis buffer [50 mM Tris/HCl (pH 7.4); 100 mM NaCl; 50 mM NaF; 5 mM EDTA; 40 mM glycerophosphate; 1 mM sodium orthovanadate; 100 μM PMSF; 1 μM leupeptin; 1 μM pepstatin A; 1% (v/v) Triton X-100] and incubated for 20 min on ice, and clarified by centrifugation at 13000 g for 5 min at 4°C.
They were then lysed in a protein kinase lysis buffer [50 mM HEPES, pH 7.5, 150 mM NaCI, 1.5 mM MgCI2, 1 mM EGTA, 1 mM sodium vanadate, 50 mM sodium fluoride, 20 mM beta-glycerophosphate, 10% (vol/vol) glycerol, 1% (vol/vol) Triton X-100, and protease inhibitors (1 mM PMSF, 1 ug/ml pepstatin A1, 1 ug/ml leupeptin, and 1 ug/ml aprotinin].
Cell lysates for immunoprecipitation were prepared by disrupting cells in immunoprecipitation buffer (20 mM Tris/HCl, pH 7.4; 150 mM NaCl; 0.5 % Triton X-100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 5 μg/ml aprotinin, 1 mM PMSF).
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