Words similar to pepstatin

Example sentences for: pepstatin

How can you use “pepstatin” in a sentence? Here are some example sentences to help you improve your vocabulary:

Next, the cells were resuspended at 3 × 10 6cells/ml in ice cold sonication buffer (250 mM sucrose; 10 mM HEPES, pH 7.4; 1 mM EDTA; 5 mM MgCl 2 ; 1 mM DTT; 0.1 mM GTP; 1 μg/ml leupeptin, 1 μg/ml pepstatin A, and 1 μg/ml aprotinin; 1 mM PMSF) and sonicated for 10 seconds.
Cell lysates for immunoprecipitation were prepared in IP-LB (25 mM Tris, pH 7.4, 150 mM NaCl, 0.5 % Triton X100, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin).
The SPM proteins were first incubated with 50 μl of protein G-agarose in buffer-1 (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, 2 μg/ml aprotinin, 5 mM EGTA, 4 mM Pefabloc SC, 1 μM pepstatin and 10 μg/ml leupeptin) at 4°C for 3 hours on a rocking platform to reduce background caused by non-specific absorption of irrelevant cellular proteins.
Protein A, the protease inhibitors phenylmethyl-sulfonyl fluoride, leupeptin and pepstatin, and 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) were all from Sigma, St. Louis, MO.
Oocytes were prepared after the method of Rho et al . [ 29 ] . Typically 30 oocytes were lysed by trituration in 200 μL of a solution containing 100 mM Tris-HCl, 100 mM NaCl, 0.5% Triton X-100, 0.05 mg/mL pepstatin, 0.05 mg/mL leupeptin, and 0.05 mg/mL aprotinin (pH 8.0) and the homogenate was incubated for 15 minutes on ice.