Words similar to p-mbp

Example sentences for: p-mbp

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Carboxyl-terminal truncation modestly increases Ppt1p activity toward both 32P-MBP and 32P-casein, and does not prevent further lipid stimulation of 32P-MBP dephosphorylation.
Like PP5, the dephosphorylation of 32P-MBP by Ppt1p was stimulated by arachidonic acid and other unsaturated fatty acids, but lipids lacking either a double bond or a carboxylate group were not effective (Table 1).
Like PP5, the control activity of Ppt1p is low toward the commonly used artificial substrates such as 32P-casein and 32P-MBP compared to other PPP enzymes [ 18 ] . Thus, it is not clear whether the selective lipid stimulation of Ppt1p toward 32P-MBP reflects a substrate-directed effect, or whether 32P-casein is simply a poor substrate for Ppt1p.
In the case of mammalian PP5, results from lipid stimulation and binding, limited proteolysis and truncation studies taken together suggest the TPR domain and the C terminus inhibit PP5 activity in a coordinated manner and that lipid binding to the TPR domain activates the enzyme in vitro [ 18 19 20 ] . In the present study we used assay conditions for Ppt1p identical to those in which PP5 was shown to be activated by removal of the TPR or C-terminal regions [ 19 ] . Under these conditions Ppt1p is also stimulated by lipids toward 32P-MBP, and activating lipids slow TPR domain proteolysis.
Lipid did not stimulate Ppt1p (1-504) toward 32P-casein, but still increased activity with 32P-MBP 12-fold.