Words similar to p-mbp

Example sentences for: p-mbp

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A similar change in susceptibility to proteolysis upon lipid binding was observed with PP5 [ 19 ] . As with PP5, only lipids that stimulated 32P-MBP dephosphorylation slowed degradation of the 20 kDa TPR fragment (Table 1).
Ppt1p activity toward 32P-MBP was stimulated by arachidoyl coenzyme A in a concentration range similar to that observed for AA, with half-maximal stimulation occurring at approximately 50 μM for arachidoyl coenzyme A and 75 μM for AA.
Like PP5, the dephosphorylation of 32P-MBP by Ppt1p was stimulated by arachidonic acid and other unsaturated fatty acids, but lipids lacking either a double bond or a carboxylate group were not effective (Table 1).
Removal of the TPR domain from Ppt1p by trypsin treatment had no effect on its activity toward 32P-casein, but increased activity toward 32P-MBP approximately two fold (Fig.
Like PP5, the control activity of Ppt1p is low toward the commonly used artificial substrates such as 32P-casein and 32P-MBP compared to other PPP enzymes [ 18 ] . Thus, it is not clear whether the selective lipid stimulation of Ppt1p toward 32P-MBP reflects a substrate-directed effect, or whether 32P-casein is simply a poor substrate for Ppt1p.