Words similar to p-mbp
Example sentences for: p-mbp
How can you use “p-mbp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Compared to full-length enzyme Ppt1p (1-504) exhibited a two-fold increase in control specific activity with both 32P-casein and 32P-MBP.
In the case of mammalian PP5, results from lipid stimulation and binding, limited proteolysis and truncation studies taken together suggest the TPR domain and the C terminus inhibit PP5 activity in a coordinated manner and that lipid binding to the TPR domain activates the enzyme in vitro [ 18 19 20 ] . In the present study we used assay conditions for Ppt1p identical to those in which PP5 was shown to be activated by removal of the TPR or C-terminal regions [ 19 ] . Under these conditions Ppt1p is also stimulated by lipids toward 32P-MBP, and activating lipids slow TPR domain proteolysis.
Ppt1p activity toward 32P-MBP was stimulated by arachidoyl coenzyme A in a concentration range similar to that observed for AA, with half-maximal stimulation occurring at approximately 50 μM for arachidoyl coenzyme A and 75 μM for AA.
A similar change in susceptibility to proteolysis upon lipid binding was observed with PP5 [ 19 ] . As with PP5, only lipids that stimulated 32P-MBP dephosphorylation slowed degradation of the 20 kDa TPR fragment (Table 1).
Like PP5, the control activity of Ppt1p is low toward the commonly used artificial substrates such as 32P-casein and 32P-MBP compared to other PPP enzymes [ 18 ] . Thus, it is not clear whether the selective lipid stimulation of Ppt1p toward 32P-MBP reflects a substrate-directed effect, or whether 32P-casein is simply a poor substrate for Ppt1p.
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