Words similar to p-mbp
Example sentences for: p-mbp
How can you use “p-mbp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Compared to full-length enzyme Ppt1p (1-504) exhibited a two-fold increase in control specific activity with both 32P-casein and 32P-MBP.
Lipid did not stimulate Ppt1p (1-504) toward 32P-casein, but still increased activity with 32P-MBP 12-fold.
Ppt1p activity toward 32P-MBP was stimulated by arachidoyl coenzyme A in a concentration range similar to that observed for AA, with half-maximal stimulation occurring at approximately 50 μM for arachidoyl coenzyme A and 75 μM for AA.
Others have proposed that fatty acyl coenzyme A esters are potential physiologic regulators of PP5, since these compounds stimulate pNPP hydrolysis by PP5 in vitro at physiologically relevant concentrations and are approximately 10-fold more potent than their free fatty acid counterparts [ 21 ] . Although we confirmed this observation using PP5 and pNPP (data not shown), in our hands similar levels of AA and arachidoyl coenzyme A were required for half-maximal stimulation of 32P-MBP dephosphorylation in the case of both Ppt1p and PP5.
In contrast, C-terminal truncation of PP5 activates the enzyme as extensively as lipid treatment, and little further activation is seen with lipid [ 19 ] . In the case of PP5, subtilisin digestion removed both the TPR domain and the C terminus [ 19 ] . The observation that C-terminal truncation increased Ppt1p activity toward both 32P-casein and 32P-MBP, but subtilisin digestion increased activity only toward 32P-MBP (see Fig.