Words similar to p-mbp
Example sentences for: p-mbp
How can you use “p-mbp” in a sentence? Here are some example sentences to help you improve your vocabulary:
Like PP5, the dephosphorylation of 32P-MBP by Ppt1p was stimulated by arachidonic acid and other unsaturated fatty acids, but lipids lacking either a double bond or a carboxylate group were not effective (Table 1).
A similar change in susceptibility to proteolysis upon lipid binding was observed with PP5 [ 19 ] . As with PP5, only lipids that stimulated 32P-MBP dephosphorylation slowed degradation of the 20 kDa TPR fragment (Table 1).
Compared to full-length enzyme Ppt1p (1-504) exhibited a two-fold increase in control specific activity with both 32P-casein and 32P-MBP.
Others have proposed that fatty acyl coenzyme A esters are potential physiologic regulators of PP5, since these compounds stimulate pNPP hydrolysis by PP5 in vitro at physiologically relevant concentrations and are approximately 10-fold more potent than their free fatty acid counterparts [ 21 ] . Although we confirmed this observation using PP5 and pNPP (data not shown), in our hands similar levels of AA and arachidoyl coenzyme A were required for half-maximal stimulation of 32P-MBP dephosphorylation in the case of both Ppt1p and PP5.
In the case of mammalian PP5, results from lipid stimulation and binding, limited proteolysis and truncation studies taken together suggest the TPR domain and the C terminus inhibit PP5 activity in a coordinated manner and that lipid binding to the TPR domain activates the enzyme in vitro [ 18 19 20 ] . In the present study we used assay conditions for Ppt1p identical to those in which PP5 was shown to be activated by removal of the TPR or C-terminal regions [ 19 ] . Under these conditions Ppt1p is also stimulated by lipids toward 32P-MBP, and activating lipids slow TPR domain proteolysis.
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