Words similar to p-mbp

Example sentences for: p-mbp

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Ppt1p activity toward 32P-MBP was stimulated by arachidoyl coenzyme A in a concentration range similar to that observed for AA, with half-maximal stimulation occurring at approximately 50 μM for arachidoyl coenzyme A and 75 μM for AA.
A similar change in susceptibility to proteolysis upon lipid binding was observed with PP5 [ 19 ] . As with PP5, only lipids that stimulated 32P-MBP dephosphorylation slowed degradation of the 20 kDa TPR fragment (Table 1).
Removal of the TPR domain from Ppt1p by trypsin treatment had no effect on its activity toward 32P-casein, but increased activity toward 32P-MBP approximately two fold (Fig.
In the case of mammalian PP5, results from lipid stimulation and binding, limited proteolysis and truncation studies taken together suggest the TPR domain and the C terminus inhibit PP5 activity in a coordinated manner and that lipid binding to the TPR domain activates the enzyme in vitro [ 18 19 20 ] . In the present study we used assay conditions for Ppt1p identical to those in which PP5 was shown to be activated by removal of the TPR or C-terminal regions [ 19 ] . Under these conditions Ppt1p is also stimulated by lipids toward 32P-MBP, and activating lipids slow TPR domain proteolysis.
Like PP5, the dephosphorylation of 32P-MBP by Ppt1p was stimulated by arachidonic acid and other unsaturated fatty acids, but lipids lacking either a double bond or a carboxylate group were not effective (Table 1).