Words similar to p-mbp
Example sentences for: p-mbp
How can you use “p-mbp” in a sentence? Here are some example sentences to help you improve your vocabulary:
AA did not stimulate 32P-MBP dephosphorylation by Ppt1p after removal of the TPR domain (data not shown).
In the case of mammalian PP5, results from lipid stimulation and binding, limited proteolysis and truncation studies taken together suggest the TPR domain and the C terminus inhibit PP5 activity in a coordinated manner and that lipid binding to the TPR domain activates the enzyme in vitro [ 18 19 20 ] . In the present study we used assay conditions for Ppt1p identical to those in which PP5 was shown to be activated by removal of the TPR or C-terminal regions [ 19 ] . Under these conditions Ppt1p is also stimulated by lipids toward 32P-MBP, and activating lipids slow TPR domain proteolysis.
Removal of the TPR domain from Ppt1p by trypsin treatment had no effect on its activity toward 32P-casein, but increased activity toward 32P-MBP approximately two fold (Fig.
Ppt1p activity toward 32P-MBP was stimulated by arachidoyl coenzyme A in a concentration range similar to that observed for AA, with half-maximal stimulation occurring at approximately 50 μM for arachidoyl coenzyme A and 75 μM for AA.
Lipid did not stimulate Ppt1p (1-504) toward 32P-casein, but still increased activity with 32P-MBP 12-fold.
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