Words similar to overexpress
Example sentences for: overexpress
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A reporter construct containing the basal promoter of the rat RIIβ 5'-flanking region (-394 to -123) upstream of a CAT-reporter gene (pCATbasic; Promega, Madison, WI) was used for assaying RIIβ promoter activity [ 18 ] . Expression vectors for USF1, USF2a and USF2b inserted 3' of the CMV promoter in pCR™3 (Invitrogen, Groningen, The Netherlands) were used to overexpress USF isoforms [ 12 ] . C/EBPβ LAP (full-length) and LIP (DNA-binding domain) expression vectors created in pCMV™3 (Stratagene, La Jolla, CA) were appreciated gifts from Dr. Shizua Akira (Hyogo College of Medicine, Hyogo, Japan).
MCF-7 cells are known to have fewer than five copies of the Her2/neu gene, whereas HCC1954 cells overexpress the protein because of gene amplification [ 40 41 ] . Her2/neu protein levels were determined with a Her2/neu ELISA kit (Oncogene) in accordance with the manufacturer's instructions.
Previously, we generated transgenic mice that overexpress the homeobox transcription factor Hoxc-8 in the thoracic region, where Hoxc-8 is normally expressed ( [ 1 ] and unpublished results).
TTP-deficient mice exhibit cachexia, with dermatitis, medullary and extramedullary hyperplasia, erosive polyarthritis, and autoimmunity with autoantibodies [ 25 ] . This phenotype is similar to that observed in mice that overexpress TNF [ 26 ] . In agreement with these data, TTP-deficient mice were shown to overproduce TNF, and weekly injections of a neutralizing antibody to TNF eliminated the predominant features of this phenotype.
Localization of this protein to the nucleus also mirrors that of endogenous PP1α in vivo . When using this system to forcibly overexpress 6His-HA-PP1α, there is a concomitant decrease in endogenous PP1α levels, suggesting the existence of an autoregulatory mechanism by which PP1α protein levels remain constant.
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