Example sentences for: oligonucleotide-directed

How can you use “oligonucleotide-directed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DHBV RNAseH activity could not be detected using yeast-derived recombinant P in either the oligonucleotide-directed RNAseH assay or the synthetic homopolymer assay, although RNAseH activity of the wild-type TY retrotransposon particles could be detected (data not shown).

  • To exclude the possibility that the DHBV RNAseH simply could not recognize the specific heteroduplex formed by DRF+ RNA and oligonucleotide D2507-, a second pair oligonucleotides (D2453+ and D2543M-Sal) were employed with DRF+ RNA in oligonucleotide-directed RNAseH assays employing permeabilized DHBV core particles.

  • The first region selected for this oligonucleotide-directed RNAseH assay was positive sense RNA derived from DHBV nt 2401 to 2605 (DRF+), which contains DR2, DR1, and the 5' half of ∈. This RNA was chosen as it contains the region where the DHBV RNAseH activity likely initiates during reverse transcription.

  • Viral cores prepared from LMH cells transfected with DHBV, DHBV-D715V, and DHBV-YMHA were employed with DRF+ RNA in the oligonucleotide-directed RNAseH assay in the first attempt to detect DHBV RNAseH activity on exogenous substrates.

  • Oligonucleotide-directed RNAseH assays with alternate substrates


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