Example sentences for: oligonucleotides

How can you use “oligonucleotides” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The 5'-end of the sagA/pel mRNA was determined by the extension of the 5'-end 32P-labeled oligonucleotides 5'-ACCTTATTTTAAAAATAAAGTTAA-3' following the method of Sambrook [ 45 ] . Oligonucleotides were labeled with [γ- 32P] ATP (10 mCi/mL in aqueous solution) (Amersham, Arlington Heights, IL) and T4 polynucleotide kinase (Gibco BRL Life Technologies, Rockville, MD).

  • The following oligonucleotides were synthesized and used as primers: PI; 5'-CCTAGTACCCTACCAGCTCA-3' P2; 5'-ATCTCCTCTTCTTGCTCTGG-3' (P1-P2; 474 bp)

  • The double stranded oligos used were as follows: for the Ets binding site on the CD5 promoter, 5' CGC GTA CAG GGA GGA AGT TGA CCT CGA 3'; for the oligonucleotides containing the CD5X region, 5' AGG CCT AAG TTG ACA GTT CAA CTT CAA ACA CTC GAG 3'; and for the CD5Y oligo, 5' AGG CCT CAC AGG CCC ACA CTG CCT GCT TCC CTG GAG 3'.

  • Ten separate malaria ORF predictions were arbitrarily selected for analysis and for each of these ORFs a set of ten oligonucleotides were synthesized.

  • The assay for the L91 locus was based on oligonucleotides specific for the L91 flanking sequence (AGGCACACAAGCCCAAAAAGAC-3') and the RB of the T-DNA [ 27 ] . PCR fragments representing the structure of the E82 locus were made as follows: Fragment 1 in Fig.


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