Words similar to oligodeoxyribonucleotides
Example sentences for: oligodeoxyribonucleotides
How can you use “oligodeoxyribonucleotides” in a sentence? Here are some example sentences to help you improve your vocabulary:
For example, oligodeoxyribonucleotide probes can be covalently attached to a surface [ 10 ] , synthesized in situ [ 11 12 13 ] , or retained via electrostatic interactions with a positively charged surface [ 14 ] . A recent study examining the effect of mismatches along the length of in situ synthesized 60 mer oligodeoxyribonucleotides demonstrated a lack of an effect of mismatches for the first ten to fifteen bases at the 3' (surface) end of the oligodeoxyribonucleotide, suggesting that these bases may not be accessible during the hybridization reaction [ 12 ] . Other problems may exist when oligodeoxyribonucleotides are adsorbed to a positively-charged surface.
Early studies with high density arrays suggested the use of 25 mer in situ synthesized oligodeoxyribonucleotides [ 1 ] . A more recent study using covalently attached oligodeoxyribonucleotides found that 30- and 35 mer oligodeoxyribonucleotides generated signals two- to five-fold higher than 25 mers, and signals obtained form 60 mers were only two-fold higher than those obtained from 30 mers but ten-fold higher than those obtained from 25 mers [ 24 ] . In fact, in situ synthesized 60 mer oligodeoxyribonucleotides, hybridized in 30-32% formamide, have been shown to represent a compromise between maximal sensitivity and specificity compared to other lengths and hybridization conditions [ 12 ] . Lastly, a study employing
An early study on DNA microarray hybridization found that hybridization was strongly dependent on the rate constants for DNA adsorption/desorption in the non-probe covered regions of the surface, the two-dimensional diffusion coefficient, and the size of probes and targets and also suggested that sparse probe coverage may provide results equal to or better than those obtained with a surface totally covered with DNA probes [ 19 ] . A theoretical analysis of the kinetics of DNA hybridization demonstrated that diffusion was important in determining the time of required to reach equilibrium and was proportional to the equilibrium binding constant and to the concentration of binding sites [ 20 ] . A recent theoretical and experimental analysis of competitive hybridization in a two-color system demonstrated the need for the hybridization kinetics of the two probes to be the same [ 21 ] . An elegant study on the use of hybridization kinetics to differentiate specific from non-specific binding was recently published [ 22 ] . This study found that the hybridization kinetics for specific and non-specific binding of labeled cRNA to surface-bound oligodeoxyribonucleotides were significantly different, with specific binding requiring longer to reach hybridization equilibrium than non-specific binding.
This property was exploited to estimate and correct for the level of hybridization contributed by non-specific binding, enabling the selection of optimal oligodeoxyribonucleotides and the reduction of false positives in exon identification.
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