Words similar to nucleolus
Example sentences for: nucleolus
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BLM has been localized to two GC-rich repeated regions (nucleolus and telomeres) [ 20] where the replication machinery is likely to need assistance to back up in order to untangle stalled forks and resolve inappropriate invasive events between repeated sequence elements found on multiple chromosomes.
Furthermore, in all (including late anaphase) cdc5-1 cells, Cdc14 co-localized with A190, a nucleolar protein (Figure 1, Rows 3 and 6), suggesting that when CDC5 is compromised, most or all Cdc14 fails to vacate the nucleolus in late anaphase.
First, net1(7m) and net1(19m') mutants exerted a modest effect on release of Cdc14 from the nucleolus, in that ~40% of cells with long mitotic spindles retained focal localization of Cdc14 and that both net1 phospho-site mutants displayed a modest defect in 'Cdc14 early anaphase release' (FEAR; [ 26 ] ). Second, net1(19m') showed a synthetic growth defect when combined with dbf2-1 or the msd2-1 allele of CDC5 . Third, phosphorylation site mapping of Net1 purified from arrested cdc14-1 cells (WS and SC, unpublished data) revealed that at least one (S231) of the five in vivo phosphorylation sites in the first 341 amino acids was directly phosphorylated by Cdc5 in vitro . Nevertheless, because expression of neither Net1(7m) nor Net1(19m') had a major impact on exit from mitosis, any direct effect of Cdc5 on Net1 appears to be minor, and the requirement for Cdc5 in the release of Cdc14 might arise principally from phosphorylation of other release-promoting proteins.
To address whether overexpression of Cdc5 could also force Cdc14 out of the nucleolus in cells arrested at a cell cycle stage where Cdc14 is nucleolar, we arrested cells in metaphase using the microtubule polymerization inhibitor nocodazole.
It was reported that in early anaphase, Cdc14 is transiently released from the nucleolus by the action of the FEAR pathway, but quickly returns to the nucleolus unless the MEN is activated to promote sustained release of Cdc14 and subsequent exit from mitosis.
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