Words similar to nucleolus
Example sentences for: nucleolus
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GAL -driven overexpression of Dbf2/(GST-Mob1) protein kinase complex did not dislodge Cdc14 from the nucleolus (data not shown).
This explanation is unlikely to account fully for Cdc5's role in exit from mitosis, however, because (1) overexpression of Cdc5, but not Dbf2/Mob1, provoked ectopic release of Cdc14 from the nucleolus; and (2) the Dbf2/Mob1 kinase complex is at least partially activated and is correctly localized to the spindle pole body in 50% of cdc5 cells at the nonpermissive temperature [ 16 17 ] .
During exit from mitosis, a TEM1/CDC15 -dependent signal frees Cdc14 from the nucleolus and allows it to promote inactivation of Clb/Cdc28 [ 15 17 19 ] . The fact that both Net1 and Cdc14 are directly involved in exit from mitosis validates the effectiveness of the tab screen and heightens the potential value of the uncharacterized tab mutants.
Biochemical and cell biological experiments indicate that Net1 acts as part of a complex named RENT that tethers Cdc14 to the nucleolus and inhibits Cdc14 phosphatase activity, and that CDC15 and TEM1 are required for the release of Cdc14 from Net1 at the end of mitosis [ 15 16 17 43 ] . Consistent with this notion, both net1 tab 2-1(which encodes a mutant version of Net1 with presumably reduced affinity for Cdc14) and CDC14 TAB 6-1(which encodes a mutant version of Cdc14 with reduced affinity for Tab2/Net1) bypass cdc15Δ [ 15 18 ] . Both net1 tab 2-1and CDC14 TAB 6-1impinge directly on mitotic exit, suggesting that other TAB genes may encode physiological regulators and effectors of the Mitotic Exit Network.
The data can be converted to a three-dimensional model shown for the detected spots (shown as red objects) and the nucleolus (shown as a blue object) within the nucleus (outline shown in green wire-frame) (Figure 6b).
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