Example sentences for: nta

How can you use “nta” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Qiagen Ni-NTA Superflow resin was equilibrated with modified buffer B before being combined with the cell lysate.

  • After centrifugation at maximum speed for 15 minutes, the supernatant was incubated with Ni-NTA resin (Qiagen) for 1.5 hour at room temperature.

  • For in vitro binding studies, about 5 μg of (His) 6 P protein was bound to 25 μl (packed volume) of Ni +2-charged NTA column using the binding buffer provided by the manufacturer (Novagen, Madison, WI).

  • The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).

  • The λ repressor fragments were purified by nickel NTA affinity chromatography (Qiagen).


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