Words similar to nta

Example sentences for: nta

How can you use “nta” in a sentence? Here are some example sentences to help you improve your vocabulary:

The supernatant fraction was diluted 1:1 with Buffer C (Buffer A containing 0.5% NP-40 and 0.5% deoxycholate, with no SDS) and loaded on to a Ni-NTA spin column (Qiagen).
The λ repressor fragments were purified by nickel NTA affinity chromatography (Qiagen).
GST-T7-Cdc14 (RDB 872) and GST-Cdc14 (RDB 1183) were expressed in E. coli BL21(DE3) pLysS cells, and purified on glutathione-agarose beads (Sigma) as described previously [ 33 ] . His6-T7-Net1(1-341) (Net1N, RDB1153) [ 11 ] was expressed in E. coli BL21(DE3) pLysS cells, and purified on Ni-NTA agarose beads (Qiagen) as described previously for p28 Kix1 [ 34 ] . Purification of His6-Plx1, His6-Plx1(N172), and His6-HA-Cdc5 from baculovirus-infected insect cells followed an earlier protocol [ 24 ] . The concentrations of purified proteins were in the ranges of: Net1N~0.
Recombinant His-tagged P protein was immobilized on a Ni-NTA column and purified recombinant human profilin was passed through it.
For in vitro binding studies, about 5 μg of (His) 6 P protein was bound to 25 μl (packed volume) of Ni +2-charged NTA column using the binding buffer provided by the manufacturer (Novagen, Madison, WI).