Example sentences for: nta
How can you use “nta” in a sentence? Here are some example sentences to help you improve your vocabulary:
The λ repressor fragments were purified by nickel NTA affinity chromatography (Qiagen).
Recombinant V80R and A61D mutants were expressed in E. Coli BL21 (DE3) pLysS cells (Novagen), and purified by Ni-NTA (Qiagen) and HiTrap SP (Pharmacia) affinity column chromatography followed by ammonium sulfate precipitation as described by Kim et al [ 7 ] .
For in vitro binding studies, about 5 μg of (His) 6 P protein was bound to 25 μl (packed volume) of Ni +2-charged NTA column using the binding buffer provided by the manufacturer (Novagen, Madison, WI).
Recombinant His-tagged P protein was immobilized on a Ni-NTA column and purified recombinant human profilin was passed through it.
After centrifugation at maximum speed for 15 minutes, the supernatant was incubated with Ni-NTA resin (Qiagen) for 1.5 hour at room temperature.
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