Example sentences for: nss
How can you use “nss” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells formed NSs during stages 1 and 2. After 2 wk, we transferred 40–80 NSs containing a total of approximately 2–4 × 10 5 cells to single wells in plates coated with 0.005% poly-L-ornithine (Sigma) and 3 μg/ml fibronectin (Invitrogen).
Thus, the sequence of gene expression accompanying formation of IPCs from NSs was similar but not identical to that described for differentiating pancreatic β-cells, and further molecular studies are required to determine the degree of endoderm-like differentiation of cells in IPCs.
1 , were expressed at low or undetectable levels in stage 1 (see Figure 1B–1C), consistent with the reported multipotency of proliferating NSs grown in high glucose [14].
Undifferentiated NSs can be readily expanded through at least a dozen passages [14], suggesting sufficient cell numbers could be generated to “scale up” this protocol for transplant-based therapies.
Thus, we postulated that glucose reduction might blunt neurogenic programs by NSs and promote alternate fates, including development of IPCs.
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