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Example sentences for: nmr
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No toxicity to maize root tissues was observed by in vivo 31P NMR spectroscopy of root tips or segments exposed to the perfusion medium for several hours, and the growth of excised root tips and roots of intact seedlings was the same over 24 h in this medium as in medium without Gd or EDTA.
All crystallographically characterized metallo-β-lactamases have a flexible amino acid chain that extends over the active site [ 37 42 44 45 46 47 48 49 ] . Previous NMR studies on CcrA have shown that this loop "clamps down" on substrate or inhibitor upon binding, and there is speculation that the distortion of substrate upon clamping down of the loop may drive catalysis [ 50 ] . The crystal structure of L1 showed that there is a large loop that extends over the active site, and modeling studies have predicted that two residues, Ile164 and Phe158, make significant contacts with large, hydrophobic substituents at the 2' or 6' positions on penicillins, cephalosporins, or carbapenems [ 37 ] . To test this prediction, Ile 164 and Phe158 were changed from large, hydrophobic residues to alanines to afford the I164A and F158A mutants.
Figure 4shows those positions in the N-terminal PH domain of pleckstrin that have been implicated, based on NMR analysis, to contact PIP 2 [ 17 ] . Boi1's PH domain has identical or similar amino acids at most of the analogous positions (Fig.
Crude products were purified by silica gel column chromatography using chloroform:hexane (7:3) to give 22 in 77% yield [ 31P NMR 105.
The structure of individual C 2 H 2 fingers as determined by 2D NMR methods has shown that each zinc finger consists of two N-terminal short anti-parallel β sheets followed by an α helix.
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