Words similar to nfs

Example sentences for: nfs

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We asked the following questions: 1) are the immuno-labeled structures within the nucleus or just closely associated; 2) is the phosphoepitope labeled by SMI-31 and RT-97 mABs specific to NFs or can it be identified on other IFs in other cell types; and 3) is there a relationship between the cell cycle as determined by DNA synthesis and the amount of nuclear labeling by SMI-31 and RT-97?
Electrophoretic and western analysis of SH-SY5Y and F98 cell extracts corroborated the finding that SMI-31 recognized an epitope on proteins other than NFs.
F98 glioma cells are not known to express neurofilament protein, and SMI-32, a mAB characterized as specific for non-phosphorylated NFs [ 19 ] , stained the expected cytoplasmic network in SH-SY5Y cells (Figure 1C) but did not label any structures in F98 glioma cells (data not shown).
Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
Alternatively, it could be that lamins or other nuclear proteins have a phosphorylated epitope also found on NFs.