Words similar to nfs
Example sentences for: nfs
How can you use “nfs” in a sentence? Here are some example sentences to help you improve your vocabulary:
F98 glioma cells are not known to express neurofilament protein, and SMI-32, a mAB characterized as specific for non-phosphorylated NFs [ 19 ] , stained the expected cytoplasmic network in SH-SY5Y cells (Figure 1C) but did not label any structures in F98 glioma cells (data not shown).
Alternatively, it could be that lamins or other nuclear proteins have a phosphorylated epitope also found on NFs.
Both RT-97 and SMI-31 may recognize the phosphorylated tyrosine motif, LNDRFAG Y IDKVR, which occurs in NF-H and thus provides the basis for the claim of these antibodies being specific for phosphorylated NFs [ 14 ] . However, this same motif occurs in GFAP and lamins A, B, and C and possibly other proteins, and is not restricted to neurons as shown by the glial staining reported here and in other studies [ 1 ] . It would be interesting to look at nuclei from a variety of tissue types using these antibodies as probes.
Electrophoretic and western analysis of SH-SY5Y and F98 cell extracts corroborated the finding that SMI-31 recognized an epitope on proteins other than NFs.
The phosphoepitope labeled by SMI-31 and RT-97 is not specific to NFs, and it can be identified on other IFs in other cell types