Words similar to nfm-pbs
Example sentences for: nfm-pbs
How can you use “nfm-pbs” in a sentence? Here are some example sentences to help you improve your vocabulary:
Target was coated on MaxiSorp plates and blocked with NFM-PBS as described [ 3 28 ] . HSPLs (phage clones or synthetic peptides) are added in the presence of varying concentrations of competitor (e.g.
The next day, unbound antigen is removed and the coated wells are blocked with 300 μl of 2% non-fat milk in PBS (NFM-PBS) for one hour at room temperature.
In all cases, the wells are blocked with NFM-PBS for 1 hour at room temperature.
The precipitant is centrifuged at 10,000 RPM at 4°C for 10 minutes and the precipitated phage pellet resupended in about 1 ml of NFM-PBS.
The phage libraries (in NFM-PBS) are thawed and 100 μl of each library is added to the antigen-coated wells and the plates are incubated for 3 hours at room temperature.