Example sentences for: nfm-pbs

How can you use “nfm-pbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Target was coated on MaxiSorp plates and blocked with NFM-PBS as described [ 3 28 ] . HSPLs (phage clones or synthetic peptides) are added in the presence of varying concentrations of competitor (e.g.

  • The next day, unbound antigen is removed and the coated wells are blocked with 300 μl of 2% non-fat milk in PBS (NFM-PBS) for one hour at room temperature.

  • In all cases, the wells are blocked with NFM-PBS for 1 hour at room temperature.

  • The precipitant is centrifuged at 10,000 RPM at 4°C for 10 minutes and the precipitated phage pellet resupended in about 1 ml of NFM-PBS.

  • The phage libraries (in NFM-PBS) are thawed and 100 μl of each library is added to the antigen-coated wells and the plates are incubated for 3 hours at room temperature.


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